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PXD018865-1

PXD018865 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMouse Muscle Stem Cell Proteome during Activation and CPEB1 Dependent Proteome in Mouse Muscle Stem Cell
DescriptionMuscle Stem Cells or satellite cells (SCs) are required for muscle regeneration. In resting muscles, SCs are kept in quiescence. After injury, SCs undergo rapid activation, proliferation and differentiation to repair damaged muscles. The transcriptome alteration during SC activation is well characterized. While transcriptome is not exactly represent proteome because of post-transcriptional regulations such as miRNA induced gene silencing. However, little is known about SC proteome. We obtained quisecent SCs (QSCs) and freshly isolated SCs (fiSCs, early activated SCs) and activated SCs (ASCs) for high resolution mass spectrometry Bruker timsTOF Pro. Thus, we uncovered the QSC proteome. By comparison of QSC proteome to fiuSCs proteome or ASCs proteome, we identified the pathways that are differentially expressed between them. Forthermore, we characterized a translational regulator CPEB1 reprogrammed translation landscape for SC activation.
HostingRepositoryPRIDE
AnnounceDate2021-11-05
AnnouncementXMLSubmission_2021-11-05_04:07:18.831.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD018865
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterTom Cheung
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListPhospho; Deamidated; Oxidation; Acetyl; Carbamidomethyl
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-28 09:30:13ID requested
12021-11-05 04:07:19announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Mouse, Muscle Stem Cell, CPEB1, Proteome, Activation
Contact List
Tom. H. CHEUNG
contact affiliationDivision of Life Science, Center for Stem Cell Research, Center of Systems Biology and Human Health, State Key Laboratory in Molecular Neuroscience, Molecular Neuroscience Center, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China. Guangdong Provincial Key Laboratory of Brain Science, Disease and Drug Development, HKUST Shenzhen Research Institute, Shenzhen-Hong Kong Institute of Brain Science, 518057, Shenzhen, Guangdong, China.
contact emailtcheung@ust.hk
lab head
Tom Cheung
contact affiliationHong Kong University of Science and Technology
contact emailtcheung@ust.hk
dataset submitter
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Dataset FTP location
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