PXD018660 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Chimeric design of Pyrrolysyl-tRNA synthetase/tRNA pairs and canonical synthetase/tRNA pairs for genetic code expansion |
Description | An orthogonal aminoacyl-tRNA synthetase/tRNA pair is a key prerequisite for site-specific incorporation of unnatural amino acids. Due to its high codon suppression efficiency and full orthogonality, the pyrrolysyl-tRNA synthetase/pyrrolysyl-tRNA pair is currently the ideal system for genetic code expansion in both eukaryotes and prokaryotes. There is a pressing need to discover or engineer other fully orthogonal translation systems that allow unnatural amino acids with distinct scaffolds and functionalities to be incorporated into a wide range of living organisms efficiently. Here, through rational chimera design by transplanting the key orthogonal components from the pyrrolysine system, we create multiple chimeric tRNA synthetase/chimeric tRNA pairs, including chimera histidine, phenylalanine, and alanine systems. We further show that these engineered chimeric systems are orthogonal and highly efficient with comparable flexibility to the pyrrolysine system. In addition, the chimera phenylalanine system can incorporate a group of phenylalanine, tyrosine and tryptophan analogues efficiently in both E. coli and mammalian cells. These aromatic amino acids analogous exhibit unique properties and characteristics, including fluorescence, post-translation modification. To our knowledge, most of these molecules have never been shown to be incorporated with fully orthogonal pairs. Therefore, these chimera pairs offer the potential for incorporation of de novo unnatural amino acids into target proteins for a variety of applications. |
HostingRepository | PRIDE |
AnnounceDate | 2020-05-25 |
AnnouncementXML | Submission_2020-06-27_17:05:10.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Wenlong Ding |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-19 09:53:57 | ID requested | |
1 | 2020-05-24 22:33:03 | announced | |
⏵ 2 | 2020-06-27 17:05:11 | announced | 2020-06-28: Updated publication reference for PubMed record(s): 32572025. |
Publication List
Ding W, Zhao H, Chen Y, Zhang B, Yang Y, Zang J, Wu J, Lin S, Chimeric design of pyrrolysyl-tRNA synthetase/tRNA pairs and canonical synthetase/tRNA pairs for genetic code expansion. Nat Commun, 11(1):3154(2020) [pubmed] |
Keyword List
submitter keyword: chimeric design, genetic code expansion |
Contact List
Shixian Lin |
contact affiliation | Life Sciences Institute, Zhejiang University, Hangzhou 310058, China |
contact email | sxlin@zju.edu.cn |
lab head | |
Wenlong Ding |
contact affiliation | Life Sciences Institute, Zhejiang University |
contact email | 0618112@zju.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018660
- Label: PRIDE project
- Name: Chimeric design of Pyrrolysyl-tRNA synthetase/tRNA pairs and canonical synthetase/tRNA pairs for genetic code expansion