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PXD018659-1

PXD018659 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCell-selective proteomics segregates pancreatic cancer subtypes by extracellular proteins in tumors and circulation
DescriptionCell-selective proteomics is emerging as a powerful concept for studying heterocellular processes. However, its potential to dissect intercellular signaling has not been exploited, despite its promise to identify non-cell autonomous disease mechanisms or biomarkers. Here, we devised an improved azidonorleucine-based protein labeling, enrichment, and mass spectrometry workflow, to achieve comprehensive proteome coverage of up to >10,100 cell-selective proteins. We provide proof-of-concept for in depth cell-selective secretomics by dissecting bidirectional intercellular signaling between co-cultured primary pancreatic ductal adenocarcinoma (PDAC) cells and macrophages. In vivo, detection of extracellular proteins emerged as a unique strength compared to FACS-based methods. Our analysis reveals systematic differences of cancer cell-derived matrisome proteins between molecular PDAC subtypes in vivo, such as elevated EMT-signal sustaining proteins. Intriguingly, high levels of pre-metastatic niche formation-associated factors in the serum reflected tumor activity in circulation. Our findings highlight how cell-selective proteomics accelerates the discovery of diagnostic markers and new therapeutic targets in cancer.
HostingRepositoryPRIDE
AnnounceDate2022-08-16
AnnouncementXMLSubmission_2022-08-15_16:30:53.573.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMario Oroshi
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF; Orbitrap Exploris 480; Q Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-19 09:53:27ID requested
12022-08-15 16:30:54announced
Publication List
Phulphagar K, K, ΓΌ, hn LI, Ebner S, Frauenstein A, Swietlik JJ, Rieckmann J, Meissner F, Proteomics reveals distinct mechanisms regulating the release of cytokines and alarmins during pyroptosis. Cell Rep, 34(10):108826(2021) [pubmed]
Keyword List
submitter keyword: LC-MSMS, proteomics, secretomics, cell-selective, azidonorleucine, PDAC, pancreatic cancer, extracellular matrix, mouse, serum, tumor
Contact List
Felix Meissner
contact affiliationExperimental Systems Immunology Laboratory, Max Planck Institute of Biochemistry, Martinsried, Germany Institute of Innate Immunity, Department of Systems Immunology and Proteomics, Medical Faculty, University of Bonn, Germany
contact emailmeissner@biochem.mpg.de
lab head
Mario Oroshi
contact affiliationProteomics
contact emailoroshi@biochem.mpg.de
dataset submitter
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