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PXD018591 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePeroxisomal targeting of the protein phosphatase Ptc5 via mitochondrial transit
DescriptionCorrect intracellular distribution of proteins is critical for the function of eukaryotic cells. Certain proteins are targeted to more than one cellular compartment, e.g. to mitochondria and peroxisomes. The protein phosphatase Ptc5 from Saccharomyces cerevisiae contains an N-terminal mitochondrial presequence followed by a trans-membrane domain and has been detected in the mitochondrial intermembrane space. Here we show mitochondrial transit of Ptc5 to peroxisomes. Translocation of Ptc5 to peroxisomes depended both on the C-terminal peroxisomal targeting signal (PTS1) and N-terminal cleavage by the mitochondrial inner membrane peptidase complex. Indirect targeting of Ptc5 to peroxisomes prevented deleterious effects of its phosphatase activity in the cytosol. Sorting of Ptc5 involves simultaneous interaction with import machineries of both organelles. We identify additional mitochondrial proteins with PTS1, which localize in both organelles and can increase their physical association. Thus, a tug-of-war like mechanism can influence the interaction and communication of two cellular compartments.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterTimo Glatter
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListDeamidated; Oxidation; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-15 15:55:15ID requested
12020-05-21 23:32:21announced
Publication List
Stehlik T, Kremp M, Kahnt J, Bölker M, Freitag J, Peroxisomal targeting of a protein phosphatase type 2C via mitochondrial transit. Nat Commun, 11(1):2355(2020) [pubmed]
Keyword List
submitter keyword: Peroxisome, protein phosphatase, Ptc5, Yeast
Contact List
Johannes Freitag
contact affiliationDepartment of Biology, Philipps University Marburg, Marburg, Germany
contact emailjohannes.freitag@biologie.uni-marburg.de
lab head
Timo Glatter
contact affiliationMPI Marburg
contact emailglatter@mpi-marburg.mpg.de
dataset submitter
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