PXD018591-1

PXD018591 is an original dataset announced via ProteomeXchange.

Title	Peroxisomal targeting of the protein phosphatase Ptc5 via mitochondrial transit
Description	Correct intracellular distribution of proteins is critical for the function of eukaryotic cells. Certain proteins are targeted to more than one cellular compartment, e.g. to mitochondria and peroxisomes. The protein phosphatase Ptc5 from Saccharomyces cerevisiae contains an N-terminal mitochondrial presequence followed by a trans-membrane domain and has been detected in the mitochondrial intermembrane space. Here we show mitochondrial transit of Ptc5 to peroxisomes. Translocation of Ptc5 to peroxisomes depended both on the C-terminal peroxisomal targeting signal (PTS1) and N-terminal cleavage by the mitochondrial inner membrane peptidase complex. Indirect targeting of Ptc5 to peroxisomes prevented deleterious effects of its phosphatase activity in the cytosol. Sorting of Ptc5 involves simultaneous interaction with import machineries of both organelles. We identify additional mitochondrial proteins with PTS1, which localize in both organelles and can increase their physical association. Thus, a tug-of-war like mechanism can influence the interaction and communication of two cellular compartments.
HostingRepository	PRIDE
AnnounceDate	2020-05-22
AnnouncementXML	Submission_2020-05-21_23:32:20.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018591
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Timo Glatter
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-15 15:55:15	ID requested
⏵ 1	2020-05-21 23:32:21	announced

Stehlik T, Kremp M, Kahnt J, B, ö, lker M, Freitag J, Peroxisomal targeting of a protein phosphatase type 2C via mitochondrial transit. Nat Commun, 11(1):2355(2020) [pubmed]

submitter keyword: Peroxisome, protein phosphatase, Ptc5, Yeast

Johannes Freitag
contact affiliation	Department of Biology, Philipps University Marburg, Marburg, Germany
contact email	johannes.freitag@biologie.uni-marburg.de
lab head
Timo Glatter
contact affiliation	MPI Marburg
contact email	glatter@mpi-marburg.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018591
     1. Label: PRIDE project
     2. Name: Peroxisomal targeting of the protein phosphatase Ptc5 via mitochondrial transit