PXD018591 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Peroxisomal targeting of the protein phosphatase Ptc5 via mitochondrial transit |
Description | Correct intracellular distribution of proteins is critical for the function of eukaryotic cells. Certain proteins are targeted to more than one cellular compartment, e.g. to mitochondria and peroxisomes. The protein phosphatase Ptc5 from Saccharomyces cerevisiae contains an N-terminal mitochondrial presequence followed by a trans-membrane domain and has been detected in the mitochondrial intermembrane space. Here we show mitochondrial transit of Ptc5 to peroxisomes. Translocation of Ptc5 to peroxisomes depended both on the C-terminal peroxisomal targeting signal (PTS1) and N-terminal cleavage by the mitochondrial inner membrane peptidase complex. Indirect targeting of Ptc5 to peroxisomes prevented deleterious effects of its phosphatase activity in the cytosol. Sorting of Ptc5 involves simultaneous interaction with import machineries of both organelles. We identify additional mitochondrial proteins with PTS1, which localize in both organelles and can increase their physical association. Thus, a tug-of-war like mechanism can influence the interaction and communication of two cellular compartments. |
HostingRepository | PRIDE |
AnnounceDate | 2020-05-22 |
AnnouncementXML | Submission_2020-05-21_23:32:20.xml |
DigitalObjectIdentifier | http://dx.doi.org/10.6019/PXD018591 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Timo Glatter |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | Deamidated; Oxidation; Carbamidomethyl |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-15 15:55:15 | ID requested | |
⏵ 1 | 2020-05-21 23:32:21 | announced | |
Publication List
Stehlik T, Kremp M, Kahnt J, Bölker M, Freitag J, Peroxisomal targeting of a protein phosphatase type 2C via mitochondrial transit. Nat Commun, 11(1):2355(2020) [pubmed] |
Keyword List
submitter keyword: Peroxisome, protein phosphatase, Ptc5, Yeast |
Contact List
Johannes Freitag |
contact affiliation | Department of Biology, Philipps University Marburg, Marburg, Germany |
contact email | johannes.freitag@biologie.uni-marburg.de |
lab head | |
Timo Glatter |
contact affiliation | MPI Marburg |
contact email | glatter@mpi-marburg.mpg.de |
dataset submitter | |
Full Dataset Link List
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018591
- Label: PRIDE project
- Name: Peroxisomal targeting of the protein phosphatase Ptc5 via mitochondrial transit