PXD018357-2

PXD018357 is an original dataset announced via ProteomeXchange.

Title	Inhibition of growth factor signaling prevents SARS-CoV-2 replication
Description	Caco-2 cells were mock-infected or infected with SARS-CoV-2 patient isolates (in biological quintruplicates) for one hour, washed, and incubated for 24 hours before cell harvest. Extracted proteins were digested and split to 1) carry out whole-cell proteomics of a tandem mass tag (TMT) 10-plex samples using liquid chromatography synchronous precursor selection mass spectromety (LC-SPS-MS3), or 2) use Fe-NTA phosphopeptide enrichment (achieving 98% enrichment) for phospho proteome analyses of a TMT 10-plex analyzed by LC-MS2 First 5 channels = control Last 5 channels = infected
HostingRepository	PRIDE
AnnounceDate	2020-05-15
AnnouncementXML	Submission_2020-09-25_03:11:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kevin Klann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-04 07:43:48	ID requested
1	2020-05-15 00:23:09	announced
⏵ 2	2020-09-25 03:11:02	announced	2020-09-25: Updated publication reference for PubMed record(s): 32877642.

Klann K, Bojkova D, Tascher G, Ciesek S, M, ü, nch C, Cinatl J, Growth Factor Receptor Signaling Inhibition Prevents SARS-CoV-2 Replication. Mol Cell, 80(1):164-174.e4(2020) [pubmed]

ProteomeXchange project tag: Sars-Cov-2, Covid-19

submitter keyword: TMT, SARS-CoV-2, COVID-19, Infection, Phospho

Christian Münch
contact affiliation	Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt am Main, Germany Frankfurt Cancer Institute, Frankfurt am Main, Germany Cardio-Pulmonary Institute, Frankfurt am Main, Germany
contact email	ch.muench@em.uni-frankfurt.de
lab head
Kevin Klann
contact affiliation	Universitätsklinikum Frankfurt am Main
contact email	klann@em.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD018357

PRIDE project URI

• PRIDE
  1. PXD018357
     1. Label: PRIDE project
     2. Name: Inhibition of growth factor signaling prevents SARS-CoV-2 replication