PXD018357

PXD018357 is an original dataset announced via ProteomeXchange.

Title	Inhibition of growth factor signaling prevents SARS-CoV-2 replication
Description	Caco-2 cells were mock-infected or infected with SARS-CoV-2 patient isolates (in biological quintruplicates) for one hour, washed, and incubated for 24 hours before cell harvest. Extracted proteins were digested and split to 1) carry out whole-cell proteomics of a tandem mass tag (TMT) 10-plex samples using liquid chromatography synchronous precursor selection mass spectromety (LC-SPS-MS3), or 2) use Fe-NTA phosphopeptide enrichment (achieving 98% enrichment) for phospho proteome analyses of a TMT 10-plex analyzed by LC-MS2 First 5 channels = control Last 5 channels = infected
HostingRepository	PRIDE
AnnounceDate	2020-05-15
AnnouncementXML	Submission_2020-09-25_03:11:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kevin Klann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-04 07:43:48	ID requested
1	2020-05-15 00:23:09	announced
⏵ 2	2020-09-25 03:11:02	announced	2020-09-25: Updated publication reference for PubMed record(s): 32877642.

Klann K, Bojkova D, Tascher G, Ciesek S, Münch C, Cinatl J, Growth Factor Receptor Signaling Inhibition Prevents SARS-CoV-2 Replication. Mol Cell, 80(1):164-174.e4(2020) [pubmed]

ProteomeXchange project tag: Sars-Cov-2, Covid-19

submitter keyword: TMT, SARS-CoV-2, COVID-19, Infection, Phospho

Christian Münch
contact affiliation	Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt am Main, Germany Frankfurt Cancer Institute, Frankfurt am Main, Germany Cardio-Pulmonary Institute, Frankfurt am Main, Germany
contact email	ch.muench@em.uni-frankfurt.de
lab head
Kevin Klann
contact affiliation	Universitätsklinikum Frankfurt am Main
contact email	klann@em.uni-frankfurt.de
dataset submitter

Dataset FTP location

PRIDE project URI

• PRIDE
  1. PXD018357
     1. Label: PRIDE project
     2. Name: Inhibition of growth factor signaling prevents SARS-CoV-2 replication