PXD018357 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Inhibition of growth factor signaling prevents SARS-CoV-2 replication |
Description | Caco-2 cells were mock-infected or infected with SARS-CoV-2 patient isolates (in biological quintruplicates) for one hour, washed, and incubated for 24 hours before cell harvest. Extracted proteins were digested and split to 1) carry out whole-cell proteomics of a tandem mass tag (TMT) 10-plex samples using liquid chromatography synchronous precursor selection mass spectromety (LC-SPS-MS3), or 2) use Fe-NTA phosphopeptide enrichment (achieving 98% enrichment) for phospho proteome analyses of a TMT 10-plex analyzed by LC-MS2 First 5 channels = control Last 5 channels = infected |
HostingRepository | PRIDE |
AnnounceDate | 2020-05-15 |
AnnouncementXML | Submission_2020-09-25_03:11:01.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kevin Klann |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-04 07:43:48 | ID requested | |
1 | 2020-05-15 00:23:09 | announced | |
⏵ 2 | 2020-09-25 03:11:02 | announced | 2020-09-25: Updated publication reference for PubMed record(s): 32877642. |
Publication List
Klann K, Bojkova D, Tascher G, Ciesek S, Münch C, Cinatl J, Growth Factor Receptor Signaling Inhibition Prevents SARS-CoV-2 Replication. Mol Cell, 80(1):164-174.e4(2020) [pubmed] |
Keyword List
ProteomeXchange project tag: Sars-Cov-2, Covid-19 |
submitter keyword: TMT, SARS-CoV-2, COVID-19, Infection, Phospho |
Contact List
Christian Münch |
contact affiliation | Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt am Main, Germany Frankfurt Cancer Institute, Frankfurt am Main, Germany Cardio-Pulmonary Institute, Frankfurt am Main, Germany |
contact email | ch.muench@em.uni-frankfurt.de |
lab head | |
Kevin Klann |
contact affiliation | Universitätsklinikum Frankfurt am Main |
contact email | klann@em.uni-frankfurt.de |
dataset submitter | |
Full Dataset Link List
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018357
- Label: PRIDE project
- Name: Inhibition of growth factor signaling prevents SARS-CoV-2 replication