PXD018271 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Antagonism of PP2A is an independent and conserved function of HIV-1 Vif and causes cell cycle arrest |
Description | The seminal description of cellular restriction factor APOBEC3G and its antagonism by HIV-1 Vif has underpinned two decades of research on the host-virus interaction. As well as APOBEC3G and its homologues, however, we have recently discovered that Vif is also able to degrade the PPP2R5 family of regulatory subunits of key cellular phosphatase PP2A (PPP2R5A-E) (Greenwood et al., 2016; Naamati et al., 2019). We now identify amino acid polymorphisms at positions 31 and 128 of HIV-1 Vif which selectively regulate the degradation of PPP2R5 family proteins. These residues covary across HIV-1 viruses in vivo, favouring depletion of PPP2R5A-E. Through analysis of point mutants and naturally occurring Vif variants, we further show that degradation of PPP2R5 family subunits is both necessary and sufficient for Vif-dependent G2/M cell cycle arrest. Antagonism of PP2A by HIV-1 Vif is therefore independent of APOBEC3 family proteins, and regulates cell cycle progression in HIV-infected cells. |
HostingRepository | PRIDE |
AnnounceDate | 2020-04-17 |
AnnouncementXML | Submission_2020-04-16_22:22:03.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | James Williamson |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-03-30 06:54:17 | ID requested | |
⏵ 1 | 2020-04-16 22:22:05 | announced | |
Publication List
Marelli S, Williamson JC, Protasio AV, Naamati A, Greenwood EJ, Deane JE, Lehner PJ, Matheson NJ, Antagonism of PP2A is an independent and conserved function of HIV-1 Vif and causes cell cycle arrest. Elife, 9():(2020) [pubmed] |
Keyword List
submitter keyword: HIV, Proteomics, PP2A, VIF |
Contact List
Nicholas J Matheson |
contact affiliation | Cambridge Institute for Therapeutic Immunology and Infectious Disease (CITIID), University of Cambridge, UK |
contact email | njm25@cam.ac.uk |
lab head | |
James Williamson |
contact affiliation | University of Cambridge |
contact email | jcw76@cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018271
- Label: PRIDE project
- Name: Antagonism of PP2A is an independent and conserved function of HIV-1 Vif and causes cell cycle arrest