PXD018271

PXD018271 is an original dataset announced via ProteomeXchange.

Title	Antagonism of PP2A is an independent and conserved function of HIV-1 Vif and causes cell cycle arrest
Description	The seminal description of cellular restriction factor APOBEC3G and its antagonism by HIV-1 Vif has underpinned two decades of research on the host-virus interaction. As well as APOBEC3G and its homologues, however, we have recently discovered that Vif is also able to degrade the PPP2R5 family of regulatory subunits of key cellular phosphatase PP2A (PPP2R5A-E) (Greenwood et al., 2016; Naamati et al., 2019). We now identify amino acid polymorphisms at positions 31 and 128 of HIV-1 Vif which selectively regulate the degradation of PPP2R5 family proteins. These residues covary across HIV-1 viruses in vivo, favouring depletion of PPP2R5A-E. Through analysis of point mutants and naturally occurring Vif variants, we further show that degradation of PPP2R5 family subunits is both necessary and sufficient for Vif-dependent G2/M cell cycle arrest. Antagonism of PP2A by HIV-1 Vif is therefore independent of APOBEC3 family proteins, and regulates cell cycle progression in HIV-infected cells.
HostingRepository	PRIDE
AnnounceDate	2020-04-17
AnnouncementXML	Submission_2020-04-16_22:22:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Williamson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-30 06:54:17	ID requested
⏵ 1	2020-04-16 22:22:05	announced

Marelli S, Williamson JC, Protasio AV, Naamati A, Greenwood EJ, Deane JE, Lehner PJ, Matheson NJ, Antagonism of PP2A is an independent and conserved function of HIV-1 Vif and causes cell cycle arrest. Elife, 9():(2020) [pubmed]

submitter keyword: HIV, Proteomics, PP2A, VIF

Nicholas J Matheson
contact affiliation	Cambridge Institute for Therapeutic Immunology and Infectious Disease (CITIID), University of Cambridge, UK
contact email	njm25@cam.ac.uk
lab head
James Williamson
contact affiliation	University of Cambridge
contact email	jcw76@cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018271
     1. Label: PRIDE project
     2. Name: Antagonism of PP2A is an independent and conserved function of HIV-1 Vif and causes cell cycle arrest