<<< Full experiment listing

PXD017983-1

PXD017983 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleImmunoprecipitation of PTEN coupled Nano-LC−ESI-MS/MS
DescriptionNuclear localization of PTEN is essential for its tumor suppressive role, and loss of nuclear PTEN is more prominent than cytoplasmic PTEN in many kinds of cancers. However, nuclear PTEN-specific regulatory mechanisms were rarely reported. Based on the finding that nuclear PTEN is more unstable than cytoplasmic PTEN. Thus, 293T cells were transiently transfected with plasmid encoding Flag-tagged PTEN along with empty vector, followed by affinity purification using an anti-Flag antibody, and the bound proteins were analyzed by liquid chromatography with tandem mass spectrometry (LC−MS/MS). Here we identify that F-box only protein 22 (FBXO22) induces ubiquitylation of nuclear but not cytoplasmic PTEN at lysine 221, which is responsible for the degradation of nuclear PTEN.
HostingRepositoryPRIDE
AnnounceDate2020-04-22
AnnouncementXMLSubmission_2020-04-22_04:44:17.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD017983
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterge mengkai
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListOxidation; Acetyl; Carbamidomethyl
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-03-11 00:35:22ID requested
12020-04-22 04:44:18announced
Publication List
Ge MK, Zhang N, Xia L, Zhang C, Dong SS, Li ZM, Ji Y, Zheng MH, Sun J, Chen GQ, Shen SM, FBXO22 degrades nuclear PTEN to promote tumorigenesis. Nat Commun, 11(1):1720(2020) [pubmed]
Keyword List
submitter keyword: 293T,PTEN,immunoprecipitation,Nano-LC−ESI-MS/MS
Contact List
Chen Guo-Qiang
contact affiliationDepartment of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Rui-Jin Hospital, and State Key Laboratory of Oncogenes and Related Genes and Chinese Academy of Medical Sciences Research Unit (NO.2019RU043), Shanghai Cancer Institute, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
contact emailchengq@shsmu.edu.cn
lab head
ge mengkai
contact affiliationSJTU
contact emailgemengk@sjtu.edu.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD017983
PRIDE project URI
Repository Record List
[ + ]