PXD017833-1

PXD017833 is an original dataset announced via ProteomeXchange.

Title	Pyruvate kinase variant of fission yeast reprograms energy metabolism triggering systemic changes in cell regulation, growth and stress resistance
Description	Cells balance glycolysis with respiration to support their energetic and biosynthetic needs in different environmental or physiological contexts. With abundant glucose, many cells prefer to grow by fermentation. Using 161 natural isolates of fission yeast, we investigated the genetic basis and phenotypic effects of the fermentation-respiration balance. The laboratory and a few other strains depended more on respiration. This trait was associated with a single-nucleotide polymorphism in a conserved region of Pyk1, the sole pyruvate kinase in fission yeast, while most organisms possess isoforms with different activity. This variant reduced Pyk1 activity and glycolytic flux. Replacing the ‘low-activity’ pyk1 allele in the laboratory strain with the ‘high-activity’ allele was sufficient to increase fermentation and decrease respiration. This metabolic reprogramming triggered systems-level adaptations in the transcriptome and proteome, and in cellular traits, including increased growth and chronological lifespan, but decreased resistance to oxidative stress. Thus, low Pyk1 activity provides no growth advantage but stress tolerance, despite increased respiration. The genetic tuning of glycolytic flux may reflect an adaptive trade-off in a species lacking pyruvate-kinase isoforms.
HostingRepository	PRIDE
AnnounceDate	2020-05-27
AnnouncementXML	Submission_2020-05-26_17:03:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Valentina Cappelletti
SpeciesList	scientific name: Schizosaccharomyces pombe 972h-; NCBI TaxID: 284812;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-02 23:30:07	ID requested
⏵ 1	2020-05-26 17:03:22	announced

Kamrad S, Grossbach J, Rodr, í, guez-L, ó, pez M, M, ü, lleder M, Townsend S, Cappelletti V, Stojanovski G, Correia-Melo C, Picotti P, Beyer A, Ralser M, B, ä, hler J, Pyruvate kinase variant of fission yeast tunes carbon metabolism, cell regulation, growth and stress resistance. Mol Syst Biol, 16(4):e9270(2020) [pubmed]

submitter keyword: fermentation, glycolysis, oxidative stress, S. pombe

Picotti Paola
contact affiliation	ETH Zurich, Institute of Molecular Systems Biology
contact email	picotti@imsb.biol.ethz.ch
lab head
Valentina Cappelletti
contact affiliation	ETH
contact email	cappelletti@imsb.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD017833

PRIDE project URI

• PRIDE
  1. PXD017833
     1. Label: PRIDE project
     2. Name: Pyruvate kinase variant of fission yeast reprograms energy metabolism triggering systemic changes in cell regulation, growth and stress resistance