PXD017729-1

PXD017729 is an original dataset announced via ProteomeXchange.

Title	Drosophila SWR1 and NuA4 complexes are defined by DOMINO isoform
Description	Histone acetylation and deposition of H2A.Z variant are integral aspects of active transcrip-tion. In Drosophila, the single DOMINO chromatin regulator complex is thought to combine both activities via an unknown mechanism. Here we show that alternative isoforms of the DOMINO nucleosome remodeling ATPase, DOM-A and DOM-B, directly specify two distinct multi-subunit complexes. Both complexes are necessary for transcriptional regulation but through different mechanisms. The DOM-B complex incorporates H2A.V (the fly ortholog of H2A.Z) genome-wide in an ATP-dependent manner, like the yeast SWR1 complex. The DOM-A complex, instead, functions as an ATP-independent histone acetyltransferase com-plex similar to the yeast NuA4, targeting lysine 12 of histone H4. Our work provides an in-structive example of how different evolutionary strategies lead to similar functional separation. In yeast and humans, nucleosome remodeling and histone acetyltransferase complexes orig-inate from gene duplication and paralog specification. Drosophila generates the same diversi-ty by alternative splicing of a single gene.
HostingRepository	PRIDE
AnnounceDate	2020-05-27
AnnouncementXML	Submission_2020-05-26_16:13:04.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alessandro Scacchetti
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	acetylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-26 00:16:16	ID requested
⏵ 1	2020-05-26 16:13:05	announced

Scacchetti A, Schauer T, Reim A, Apostolou Z, Campos Sparr A, Krause S, Heun P, Wierer M, Becker PB, SWR1 and NuA4 complexes are defined by DOMINO isoforms. Elife, 9():(2020) [pubmed]

submitter keyword: Drosophila, DOMINO, SWR1, NuA4, H3ac, H4ac

Peter B. Becker
contact affiliation	Molecular Biology Division, Biomedical Center, Ludwig-Maximilians-University, Munich, Germany
contact email	pbecker@bmc.med.lmu.de
lab head
Alessandro Scacchetti
contact affiliation	Molecular Biology Division, Biomedical Center, Ludwig-Maximilians-University, Munich
contact email	Alessandro.Scacchetti@med.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD017729

PRIDE project URI

• PRIDE
  1. PXD017729
     1. Label: PRIDE project
     2. Name: Drosophila SWR1 and NuA4 complexes are defined by DOMINO isoform