PXD017697 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Extensive protein group SUMO modification of repressive chromatin factors distinguishes pluripotent from somatic cells |
Description | Post-translational modification by SUMO is a key regulator of cell identity. In mouse embryonic fibroblasts (MEFs), SUMO impedes reprogramming to pluripotency, while in embryonic stem cells (ESCs), it represses the emergence of totipotent-like cells, suggesting that SUMO targets distinct substrates to preserve somatic and pluripotent states. Using MS-based proteomics, we show that the composition of endogenous SUMOylomes differs dramatically between MEFs and ESCs. In MEFs, SUMO2/3 targets proteins associated with canonical SUMO functions, such as splicing, and transcriptional regulators driving somatic enhancer selection. In contrast, in ESCs, SUMO2/3 primarily modifies highly interconnected repressive chromatin complexes, thereby preventing chromatin opening and transitioning to totipotent-like states. We also characterize several SUMO-modified pluripotency factors and show that SUMOylation of Dppa2 and Dppa4 impedes the conversion to 2-cell-embryo-like states. Altogether, we propose that rewiring the repertoire of SUMO target networks is a major driver of cell fate decision during embryonic development. |
HostingRepository | PRIDE |
AnnounceDate | 2020-09-16 |
AnnouncementXML | Submission_2020-09-15_23:03:45.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ivo Hendriks |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | sumoylated lysine |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-02-24 02:21:01 | ID requested | |
⏵ 1 | 2020-09-15 23:03:45 | announced | |
2 | 2020-09-25 03:16:31 | announced | 2020-09-25: Updated publication reference for PubMed record(s): 32937131. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: SUMO, endogenous, ESC, MEF, stem cells, pluripotent, somatic, chromatin |
Contact List
Michael Lund Nielsen |
contact affiliation | Proteomics program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark |
contact email | michael.lund.nielsen@cpr.ku.dk |
lab head | |
Ivo Hendriks |
contact affiliation | Novo Nordisk Foundation Center for Protein Research, University of Copenhagen |
contact email | ivo.a.hendriks@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD017697
- Label: PRIDE project
- Name: Extensive protein group SUMO modification of repressive chromatin factors distinguishes pluripotent from somatic cells