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PXD017697

PXD017697 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleExtensive protein group SUMO modification of repressive chromatin factors distinguishes pluripotent from somatic cells
DescriptionPost-translational modification by SUMO is a key regulator of cell identity. In mouse embryonic fibroblasts (MEFs), SUMO impedes reprogramming to pluripotency, while in embryonic stem cells (ESCs), it represses the emergence of totipotent-like cells, suggesting that SUMO targets distinct substrates to preserve somatic and pluripotent states. Using MS-based proteomics, we show that the composition of endogenous SUMOylomes differs dramatically between MEFs and ESCs. In MEFs, SUMO2/3 targets proteins associated with canonical SUMO functions, such as splicing, and transcriptional regulators driving somatic enhancer selection. In contrast, in ESCs, SUMO2/3 primarily modifies highly interconnected repressive chromatin complexes, thereby preventing chromatin opening and transitioning to totipotent-like states. We also characterize several SUMO-modified pluripotency factors and show that SUMOylation of Dppa2 and Dppa4 impedes the conversion to 2-cell-embryo-like states. Altogether, we propose that rewiring the repertoire of SUMO target networks is a major driver of cell fate decision during embryonic development.
HostingRepositoryPRIDE
AnnounceDate2020-09-16
AnnouncementXMLSubmission_2020-09-25_03:16:30.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIvo Hendriks
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListsumoylated lysine
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-02-24 02:21:01ID requested
12020-09-15 23:03:45announced
22020-09-25 03:16:31announced2020-09-25: Updated publication reference for PubMed record(s): 32937131.
Publication List
Theurillat I, Hendriks IA, Cossec JC, Andrieux A, Nielsen ML, Dejean A, Extensive SUMO Modification of Repressive Chromatin Factors Distinguishes Pluripotent from Somatic Cells. Cell Rep, 32(11):108146(2020) [pubmed]
Keyword List
submitter keyword: SUMO, endogenous, ESC, MEF, stem cells, pluripotent, somatic, chromatin
Contact List
Michael Lund Nielsen
contact affiliationProteomics program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark
contact emailmichael.lund.nielsen@cpr.ku.dk
lab head
Ivo Hendriks
contact affiliationNovo Nordisk Foundation Center for Protein Research, University of Copenhagen
contact emailivo.a.hendriks@gmail.com
dataset submitter
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