PXD017642 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Interactome analysis of FAM50A via affinity purification coupled to mass spectrometry |
Description | FAM50A missense variants are present in Armfield XLID syndrome patients. Zebrafish lacking fam50a display related phenotypes and show dysregulation of the transcriptome, increased spliceosome mRNAs and a preponderance of 3’ alternative splicing events, suggesting a role for Fam50a in the spliceosome C complex. Fam50A protein-protein interaction studies show that in 293T cells FAM50A interacts with spliceosome components. In sum, aberrant mRNA processing caused by FAM50A mutation underpins Armfield XLID syndrome. |
HostingRepository | PRIDE |
AnnounceDate | 2020-05-08 |
AnnouncementXML | Submission_2020-05-07_22:20:20.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | James Wright |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | 2-pyrrolidone-5-carboxylic acid (Glu); acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-02-19 23:38:12 | ID requested | |
⏵ 1 | 2020-05-07 22:20:21 | announced | |
2 | 2020-08-11 04:15:56 | announced | 2020-08-11: Updated publication reference for PubMed record(s): 32703943. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Streptavidin affinity purification, FAM50A, protein interactions, nanoLC-MS/MS |
Contact List
Jyoti Choudhary |
contact affiliation | The Institute of Cancer Research |
contact email | jyoti.choudhary@icr.ac.uk |
lab head | |
James Wright |
contact affiliation | The Institute of Cancer Research |
contact email | james.wright@icr.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017642
- Label: PRIDE project
- Name: Interactome analysis of FAM50A via affinity purification coupled to mass spectrometry