PXD017597 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparative proteomics of three Giardia lamblia strains: Antigenic variation in the post-genomic era |
Description | Giardia lamblia is a causative agent of persistent diarrhea widespread in regions with low hygienic standards. Laboratory research is based on cloned lines issuing from various patient isolates typed in the late 1980s and 90s using restriction analysis and serology. In the present study, we compared the well characterized strain WBC6 with another clone of the parent WB isolate termed WBA1 and with a clone from another isolate, GS/M-83-H7, using shotgun mass spectrometry proteomics. We identified 398 proteins differentially expressed between the GS and both WB isolates and 97 proteins differentially expressed between both WB isolates. We investigated the expression levels of the predominant variant-specific surface proteins (VSPs) in each clone and matched the previously described major VSPs of each strain to the corresponding open reading frame (ORF) sequences identified by whole-genome sequencing efforts. Furthermore, since the original WB isolate comes from a patient treated with metronidazole, we compared the susceptibilities of the strains to nitro compounds, as well the expression levels of enzymes involved in nitro reduction and on the corresponding enzyme activities and found distinct differences between the three strains. |
HostingRepository | PRIDE |
AnnounceDate | 2020-05-20 |
AnnouncementXML | Submission_2020-05-19_22:40:46.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Manfred Heller |
SpeciesList | scientific name: Giardia intestinalis; NCBI TaxID: 5741; |
ModificationList | monohydroxylated residue; acetylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-02-18 09:11:46 | ID requested | |
⏵ 1 | 2020-05-19 22:40:47 | announced | |
Publication List
M, ü, ller J, Braga S, Uldry AC, Heller M, M, ü, ller N, strains: investigation of antigenic variation in the post-genomic era. Parasitology, 147(9):1008-1018(2020) [pubmed] |
Keyword List
submitter keyword: Diversity - drug susceptibility – isolates - parasite-host-interaction |
Contact List
Loachim Müller |
contact affiliation | Institute of Parasitology, Vetsuisse Faculty, University of Berne, Länggass-Strasse 122, CH-3012 Berne, Switzerland |
contact email | joachim.mueller@vetsuisse.unibe.ch |
lab head | |
Manfred Heller |
contact affiliation | Proteomics and Mass Spectrometry Core Facility, Department for BioMedical Research (DBMR), University of Bern, Bern, Switzerland |
contact email | pmscf@dbmr.unibe.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017597
- Label: PRIDE project
- Name: Comparative proteomics of three Giardia lamblia strains: Antigenic variation in the post-genomic era