PXD017597

PXD017597 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomics of three Giardia lamblia strains: Antigenic variation in the post-genomic era
Description	Giardia lamblia is a causative agent of persistent diarrhea widespread in regions with low hygienic standards. Laboratory research is based on cloned lines issuing from various patient isolates typed in the late 1980s and 90s using restriction analysis and serology. In the present study, we compared the well characterized strain WBC6 with another clone of the parent WB isolate termed WBA1 and with a clone from another isolate, GS/M-83-H7, using shotgun mass spectrometry proteomics. We identified 398 proteins differentially expressed between the GS and both WB isolates and 97 proteins differentially expressed between both WB isolates. We investigated the expression levels of the predominant variant-specific surface proteins (VSPs) in each clone and matched the previously described major VSPs of each strain to the corresponding open reading frame (ORF) sequences identified by whole-genome sequencing efforts. Furthermore, since the original WB isolate comes from a patient treated with metronidazole, we compared the susceptibilities of the strains to nitro compounds, as well the expression levels of enzymes involved in nitro reduction and on the corresponding enzyme activities and found distinct differences between the three strains.
HostingRepository	PRIDE
AnnounceDate	2020-05-20
AnnouncementXML	Submission_2020-05-19_22:40:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manfred Heller
SpeciesList	scientific name: Giardia intestinalis; NCBI TaxID: 5741;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-18 09:11:46	ID requested
⏵ 1	2020-05-19 22:40:47	announced

M, ü, ller J, Braga S, Uldry AC, Heller M, M, ü, ller N, strains: investigation of antigenic variation in the post-genomic era. Parasitology, 147(9):1008-1018(2020) [pubmed]

submitter keyword: Diversity - drug susceptibility – isolates - parasite-host-interaction

Loachim Müller
contact affiliation	Institute of Parasitology, Vetsuisse Faculty, University of Berne, Länggass-Strasse 122, CH-3012 Berne, Switzerland
contact email	joachim.mueller@vetsuisse.unibe.ch
lab head
Manfred Heller
contact affiliation	Proteomics and Mass Spectrometry Core Facility, Department for BioMedical Research (DBMR), University of Bern, Bern, Switzerland
contact email	pmscf@dbmr.unibe.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD017597

PRIDE project URI

• PRIDE
  1. PXD017597
     1. Label: PRIDE project
     2. Name: Comparative proteomics of three Giardia lamblia strains: Antigenic variation in the post-genomic era