PXD017578-1

PXD017578 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of proteins responsive to drought and low temperature stress in a hard red spring wheat cultivar
Description	Drought stress is becoming more prevalent with global warming, and has been shown to have large effects on gluten proteins linked to wheat bread making quality. Likewise, low temperature stress can detrimentally affect proteins in wheat. This study was done to determine the differential expression of high molecular weight (HMW) glutenin proteins in a drought and low temperature stressed high quality hard red spring wheat cultivar (PAN3478), against a control. The treatments were applied in the greenhouse at the soft dough stage. HMW glutenin proteins were extracted from the flour, and separated by two-dimensional gel electrophoresis. Protein spots that had p values lower than 0.05 and fold value equal to or greater than 1.2 were considered significantly differentially expressed. These proteins were further analyzed by tandem mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2020-03-26
AnnouncementXML	Submission_2020-03-26_02:34:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vera Muccilli
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-18 02:43:57	ID requested
⏵ 1	2020-03-26 02:35:36	announced

Labuschagne M, Masci S, Tundo S, Muccilli V, Saletti R, van Biljon A, Proteomic Analysis of Proteins Responsive to Drought and Low Temperature Stress in a Hard Red Spring Wheat Cultivar. Molecules, 25(6):(2020) [pubmed]

submitter keyword: abiotic stress

bread wheat

glutenin

proteomics

Salvatore Foti
contact affiliation	Department of Chemical Sciences, Organic Mass Spectrometry Laboratory, University of Catania, Viale A. Doria 6, 95125 Catania, Italy
contact email	sfoti@unict.it
lab head
Vera Muccilli
contact affiliation	Department of Chemical Sciences, University of Catania
contact email	v.muccilli@unict.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017578
     1. Label: PRIDE project
     2. Name: Proteomic analysis of proteins responsive to drought and low temperature stress in a hard red spring wheat cultivar