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PXD017388-1

PXD017388 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleReproducible label-free quantitative proteomics profiling cellular responses to engineered nanomaterials
DescriptionProteomic data from engineered nanomaterial- treated THP-1 cells. Time points at 12 hours; 4 biological replicates for each treatment. Human THP-1 cells were either mock-treated (control) or treated with engineered nanomaterials (ENMs). Four biological replicates were used for each sample group (Control, or ENM treatment at either the low or high dosage). In the block design scheme for LC-MS analysis, samples treated with two different ENMs were assign into an analytical block (2 ENM X 2 dosages/ENM X 4 replicates = 16 samples). Four control samples were also included in each block, yielding 20 samples per block. Blocks of samples were divided into two processing batches. Samples in each batch were processed simultaneously from cell culture, to treatment with ENMs, and finally to proteomic sample preparation for LC-MS analysis (including trypsin digestion).
HostingRepositoryMassIVE
AnnounceDate2020-02-27
AnnouncementXMLSubmission_2020-02-27_14:06:39.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMatthew Monroe
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListCarbamidomethyl; Oxidation
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-02-05 00:17:42ID requested
12020-02-27 14:06:40announced
Publication List
Tong Zhang, Matthew J. Gaffrey, Dennis G. Thomas, Thomas J. Weber, Becky M. Hess, Karl K.Weitz, Paul D. Piehowski, Vladislav A. Petyuk, Ronald J. Moore, Wei-Jun Qian, and Brian D. Thrall. A proteome-wide assessment of the oxidative stress paradigm for metal and metal-oxide nanomaterials in human macrophages. NanoImpact. 2020 Jan
17: 100194. doi: 10.1016/j.impact.2019.100194.
Keyword List
submitter keyword: proteomics, label-free quantification, large cohort
Contact List
Wei-Jun Qian
contact affiliationPacific Northwest National Laboratory
contact emailWeijun.Qian@pnnl.gov
lab head
Matthew Monroe
contact affiliationPacific Northwest National Laboratory
contact emailmatthew.monroe@pnnl.gov
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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