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PXD017266-1

PXD017266 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome of Pseudomonas aeruginosa PAO1 wild-type and mutant containing a single nucleotide polymorphism in fusA1
DescriptionThe gene encoding elongation factor G, fusA1, is frequently mutated in clinical isolates of Pseudomonas aeruginosa from patients with cystic fibrosis. Recent work has shown that fusA1 mutants often display elevated aminoglycoside resistance due to increased expression of the aminoglycoside efflux pump, MexXY. We isolated a spontaneous gentamicin-resistant fusA1 mutant (FusA1-P443L) in which mexXY expression was increased. We compared the proteome of this fusA1 mutant (EMC1) with the P. aeruginosa PAO1-derived progenitor strain (EMC0) and complemented mutant strain expressing the wild-type fusA1 gene in trans (EMC1*).
HostingRepositoryPRIDE
AnnounceDate2020-09-16
AnnouncementXMLSubmission_2020-09-15_23:23:27.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEve Maunders
SpeciesList scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-01-23 06:20:39ID requested
12020-09-15 23:23:28announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Biological, Pseudomonas aeruginosa, elongation factor G, fusA1, TMT
Contact List
Martin Welch
contact affiliationDepartment of Biochemistry, University of Cambridge, UK
contact emailmw240@cam.ac.uk
lab head
Eve Maunders
contact affiliationUniversity of Melbourne
contact emaileamaunders@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
Repository Record List
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