PXD017266 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome of Pseudomonas aeruginosa PAO1 wild-type and mutant containing a single nucleotide polymorphism in fusA1 |
Description | The gene encoding elongation factor G, fusA1, is frequently mutated in clinical isolates of Pseudomonas aeruginosa from patients with cystic fibrosis. Recent work has shown that fusA1 mutants often display elevated aminoglycoside resistance due to increased expression of the aminoglycoside efflux pump, MexXY. We isolated a spontaneous gentamicin-resistant fusA1 mutant (FusA1-P443L) in which mexXY expression was increased. We compared the proteome of this fusA1 mutant (EMC1) with the P. aeruginosa PAO1-derived progenitor strain (EMC0) and complemented mutant strain expressing the wild-type fusA1 gene in trans (EMC1*). |
HostingRepository | PRIDE |
AnnounceDate | 2020-09-16 |
AnnouncementXML | Submission_2020-09-15_23:23:27.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Eve Maunders |
SpeciesList | scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-01-23 06:20:39 | ID requested | |
⏵ 1 | 2020-09-15 23:23:28 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Biological, Pseudomonas aeruginosa, elongation factor G, fusA1, TMT |
Contact List
Martin Welch |
contact affiliation | Department of Biochemistry, University of Cambridge, UK |
contact email | mw240@cam.ac.uk |
lab head | |
Eve Maunders |
contact affiliation | University of Melbourne |
contact email | eamaunders@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017266
- Label: PRIDE project
- Name: Proteome of Pseudomonas aeruginosa PAO1 wild-type and mutant containing a single nucleotide polymorphism in fusA1