PXD017185

PXD017185 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	DIA proteomics of dried blood spot proteins by sodium carbonate
Description	We developed a simple and sensitive analysis method for dried blood spot (DBS) proteins by sodium carbonate precipitation (SCP). We aimed to removing large amounts of hydrophilic blood proteins such as hemoglobin, albumin, fibrinogen and transferrin in DBS using a simple pretreatment with sodium carbonate solution, which concentrates membrane proteins. We evaluated the performance of the SCP method by comparing the LC-MS/MS results obtained for proteins prepared by SCP with those obtained by sodium dodecanate (SoDod) extract as total protein solubilized from DBS.
HostingRepository	jPOST
AnnounceDate	2020-01-20
AnnouncementXML	Submission_2023-01-20_16:52:54.990.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daisuke Nakajima
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; unknown modification
Instrument	Q Exactive HF-X

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-19 16:34:54	ID requested
1	2020-01-19 16:34:58	announced
2	2021-02-04 16:55:44	announced	2021-02-04: Updated FTP location.
3	2022-09-18 04:00:41	announced	2022-09-18: Updated FTP location.
⏵ 4	2023-01-20 16:52:55	announced	2023-01-21: Updated PubMed.

Publication List

Nakajima D, Kawashima Y, Shibata H, Yasumi T, Isa M, Izawa K, Nishikomori R, Heike T, Ohara O, Simple and Sensitive Analysis for Dried Blood Spot Proteins by Sodium Carbonate Precipitation for Clinical Proteomics. J Proteome Res, 19(7):2821-2827(2020) [pubmed]

Nakajima D, Kawashima Y, Shibata H, Yasumi T, Isa M, Izawa K, Nishikomori R, Heike T, Ohara O, Simple and Sensitive Analysis for Dried Blood Spot Proteins by Sodium Carbonate Precipitation for Clinical Proteomics. J Proteome Res, 19(7):2821-2827(2020) [pubmed]

Keyword List

submitter keyword: Dried blood spot, DIA, clinical proteomics

submitter keyword: Dried blood spot, DIA, clinical proteomics

Contact List

Daisuke Nakajima
lab head
Daisuke Nakajima
contact affiliation	Kazusa DNA Research Institute
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000735/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000735/