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PXD017159 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDynamics in protein translation sustaining T cell preparedness
DescriptionIn response to pathogenic threats, naïve T cells rapidly transition from a quiescent to activated state, yet the underlying mechanisms are incompletely understood. Using a pulsed SILAC approach, we investigated the dynamics of mRNA translation kinetics and protein turnover in human naïve and activated T cells. Our datasets uncovered that transcription factors maintaining T cell quiescence had constitutively high turnover, which facilitated their depletion upon activation. Furthermore, naïve T cells maintained a surprisingly large number of idling ribosomes as well as 242 repressed mRNA species and a reservoir of glycolytic enzymes. These components were rapidly engaged following stimulation, promoting an immediate translational and glycolytic switch to ramp up the T cell activation program. Our data elucidate new insights into how T cells maintain a prepared state to mount a rapid immune response, and provide a resource of protein turnover, absolute translation kinetics and protein synthesis rates in T cells (www.immunomics.ch).
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMatteo Pecoraro
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList6x(13)C labeled residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-01-17 03:29:57ID requested
12020-06-09 08:54:17announced
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: T cells, T cell preparedness, T cell activation
Contact List
Roger Geiger
contact affiliationInstitute for Research in Biomedicine, Università della Svizzera italiana, Switzerland
contact emailroger.geiger@irb.usi.ch
lab head
Matteo Pecoraro
contact affiliationInstitute for Research in Biomedicine, Via Vincenzo Vela 6 - CH-6500 Bellinzona
contact emailmatteo.pecoraro@irb.usi.ch
dataset submitter
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