PXD017159-1

PXD017159 is an original dataset announced via ProteomeXchange.

Title	Dynamics in protein translation sustaining T cell preparedness
Description	In response to pathogenic threats, naïve T cells rapidly transition from a quiescent to activated state, yet the underlying mechanisms are incompletely understood. Using a pulsed SILAC approach, we investigated the dynamics of mRNA translation kinetics and protein turnover in human naïve and activated T cells. Our datasets uncovered that transcription factors maintaining T cell quiescence had constitutively high turnover, which facilitated their depletion upon activation. Furthermore, naïve T cells maintained a surprisingly large number of idling ribosomes as well as 242 repressed mRNA species and a reservoir of glycolytic enzymes. These components were rapidly engaged following stimulation, promoting an immediate translational and glycolytic switch to ramp up the T cell activation program. Our data elucidate new insights into how T cells maintain a prepared state to mount a rapid immune response, and provide a resource of protein turnover, absolute translation kinetics and protein synthesis rates in T cells (www.immunomics.ch).
HostingRepository	PRIDE
AnnounceDate	2020-06-09
AnnouncementXML	Submission_2020-06-09_08:54:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matteo Pecoraro
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-17 03:29:57	ID requested
⏵ 1	2020-06-09 08:54:17	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: T cells, T cell preparedness, T cell activation

Roger Geiger
contact affiliation	Institute for Research in Biomedicine, Università della Svizzera italiana, Switzerland
contact email	roger.geiger@irb.usi.ch
lab head
Matteo Pecoraro
contact affiliation	Institute for Research in Biomedicine, Via Vincenzo Vela 6 - CH-6500 Bellinzona
contact email	matteo.pecoraro@irb.usi.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD017159

PRIDE project URI

• PRIDE
  1. PXD017159
     1. Label: PRIDE project
     2. Name: Dynamics in protein translation sustaining T cell preparedness