PXD017140 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Yeast glutamine tRNA synthetase doxycycline shut-off |
Description | During protein synthesis, charged tRNAs deliver amino acids to translating ribosomes, and are then re-charged by tRNA synthetases (aaRS). In humans, mutant aaRS cause a diversity of neurological disorders, but their molecular aetiologies are incompletely characterised. To understand system responses to aaRS depletion, the yeast glutamine aaRS gene (GLN4) was transcriptionally regulated using doxycycline by tet-off control. Depletion of Gln4p inhibited growth, and induced a transcriptional GCN4 amino acid starvation response, indicative of uncharged tRNA accumulation and Gcn2 kinase activation. The GCN4 response was confirmed using SILAC proteomics, using heavy isotope labelling to identify changes in protein expression during glutamine tRNA synthetase depletion. |
HostingRepository | PRIDE |
AnnounceDate | 2020-02-12 |
AnnouncementXML | Submission_2020-02-17_01:07:55.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ian Stansfield |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | 6x(13)C labeled residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-01-16 01:38:40 | ID requested | |
1 | 2020-02-12 03:23:18 | announced | |
⏵ 2 | 2020-02-17 01:07:56 | announced | 2020-02-17: Updated publication reference for PubMed record(s): 32016368. |
Publication List
McFarland MR, Keller CD, Childers BM, Adeniyi SA, Corrigall H, Raguin A, Romano MC, Stansfield I, The molecular aetiology of tRNA synthetase depletion: induction of a GCN4 amino acid starvation response despite homeostatic maintenance of charged tRNA levels. Nucleic Acids Res, 48(6):3071-3088(2020) [pubmed] |
Keyword List
submitter keyword: translation, tRNA synthetase depletion, Saccharomyces cerevisiae, SILAC |
Contact List
Ian Stansfield |
contact affiliation | Institute of Medical Sciences, University of Aberdeen, UK |
contact email | i.stansfield@abdn.ac.uk |
lab head | |
Ian Stansfield |
contact affiliation | University of Aberdeen |
contact email | i.stansfield@abdn.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017140
- Label: PRIDE project
- Name: Yeast glutamine tRNA synthetase doxycycline shut-off