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PXD017140

PXD017140 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleYeast glutamine tRNA synthetase doxycycline shut-off
DescriptionDuring protein synthesis, charged tRNAs deliver amino acids to translating ribosomes, and are then re-charged by tRNA synthetases (aaRS). In humans, mutant aaRS cause a diversity of neurological disorders, but their molecular aetiologies are incompletely characterised. To understand system responses to aaRS depletion, the yeast glutamine aaRS gene (GLN4) was transcriptionally regulated using doxycycline by tet-off control. Depletion of Gln4p inhibited growth, and induced a transcriptional GCN4 amino acid starvation response, indicative of uncharged tRNA accumulation and Gcn2 kinase activation. The GCN4 response was confirmed using SILAC proteomics, using heavy isotope labelling to identify changes in protein expression during glutamine tRNA synthetase depletion.
HostingRepositoryPRIDE
AnnounceDate2020-02-12
AnnouncementXMLSubmission_2020-02-17_01:07:55.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIan Stansfield
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList6x(13)C labeled residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-01-16 01:38:40ID requested
12020-02-12 03:23:18announced
22020-02-17 01:07:56announced2020-02-17: Updated publication reference for PubMed record(s): 32016368.
Publication List
McFarland MR, Keller CD, Childers BM, Adeniyi SA, Corrigall H, Raguin A, Romano MC, Stansfield I, The molecular aetiology of tRNA synthetase depletion: induction of a GCN4 amino acid starvation response despite homeostatic maintenance of charged tRNA levels. Nucleic Acids Res, 48(6):3071-3088(2020) [pubmed]
Keyword List
submitter keyword: translation, tRNA synthetase depletion, Saccharomyces cerevisiae, SILAC
Contact List
Ian Stansfield
contact affiliationInstitute of Medical Sciences, University of Aberdeen, UK
contact emaili.stansfield@abdn.ac.uk
lab head
Ian Stansfield
contact affiliationUniversity of Aberdeen
contact emaili.stansfield@abdn.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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