PXD017059

PXD017059 is an original dataset announced via ProteomeXchange.

Title	Correlation of Prevotella abundancy with a particular carbohydrate-active CAZy enzyme profile in healthy gut microbiota
Description	Gut microbiota participates in diverse metabolic and homeostatic functions related to health and well-being. Individual variation in its composition depends on many factors including dietary factors. We profiled enzymatic activity of fecal microbiota in 63 healthy adult individuals using metaproteomics, and identified Bacteroides and Prevotella –derived microbial CAZy (carbohydrate-active) enzymes involved in glycan foraging. One particular profile with many Bacteroides-derived CAZy was identified in one-third of subjects (n=20), and it associated with high abundancy of Bacteroides in most subjects. In other subjects (n=8) with dietary parameters similar to former, microbiota showed intense expression of Prevotella-derived CAZy including exo−beta−(1,4)−xylanase, xylan-1,4−beta−xylosidase, alpha−L−arabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides. This associated invariably with robust representation of Prevotella in gut microbiota, while subjects with intermediate representation of Prevotella showed no CAZy profile. Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with robust differences in microbiota composition, the latter with exceptionally high Prevotella abundancy in the gut, are in evidence of individual variation in metabolic adaptation of gut microbiota with an impact on colonizing competence.
HostingRepository	PRIDE
AnnounceDate	2021-07-01
AnnouncementXML	Submission_2021-06-30_22:24:31.185.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sami Pietila
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-10 03:58:36	ID requested
1	2021-06-30 05:56:53	announced
⏵ 2	2021-06-30 22:24:31	announced	2021-07-01: Updated project metadata.

Aakko J, Pietil, ä S, Toivonen R, Rokka A, Mokkala K, Laitinen K, Elo L, H, ä, nninen A, A carbohydrate-active enzyme (CAZy) profile links successful metabolic specialization of Prevotella to its abundance in gut microbiota. Sci Rep, 10(1):12411(2020) [pubmed]

10.1038/S41598-020-69241-2;

submitter keyword: metaproteomics, DIA

Arno Hänninen
contact affiliation	Department of Medical Microbiology and Immunology, University of Turku, Finland TYKS Microbiology, Turku University Central Hospital, Turku, Finland
contact email	arno.hanninen@utu.fi
lab head
Sami Pietila
contact affiliation	University of Turku
contact email	sampie@utu.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD017059

PRIDE project URI

• PRIDE
  1. PXD017059
     1. Label: PRIDE project
     2. Name: Correlation of Prevotella abundancy with a particular carbohydrate-active CAZy enzyme profile in healthy gut microbiota