PXD017022-1

PXD017022 is an original dataset announced via ProteomeXchange.

Title	TRF1 averts chromatin remodelling, recombination and replication dependent-Break Induced Replication at mouse telomeres
Description	Telomeric repeat sequences are prone to induce replication stress and one of the core telomere binding proteins, TRF1, is thought to counteract these problems. The molecular mechanisms coming into play upon TRF1 loss were not clearly defined though. This study combines an inducible TRF1 loss with telomere-targeted proteomics to analyze the incurring changes. The results show that loss of TRF1 causes important alterations in telomere associated proteins and the new profile resembles in a number of ways that of cells in which telomeres are maintained by telomerase-independent mechanisms, named ALT. The presence of DNA repair proteins (MRN complex, the SMC5/6 complex), some increases in telomere colocalization with PML bodies, sister chromatid exchanges and clear signs of increased TERRA transcription and mitotic break-induced replication are part of these phenotypes. The data also show that these early ALT phenotypes can be differentiated from drug induced replication stress, and part of this ALT response is conserved in human cells. These studies therefore point to a critical role for TRF1 in preventing the emergence of ALT and hence immortalization of cells. Given that the details for how ALT actually is initiated remained unclear, these findings are an important step forward and will spur new research into that question.
HostingRepository	PRIDE
AnnounceDate	2020-01-22
AnnouncementXML	Submission_2020-01-22_07:42:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alex Montoya
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-09 00:20:27	ID requested
⏵ 1	2020-01-22 07:42:10	announced

Porreca RM, Herrera-Moyano E, Skourti E, Law PP, Gonzalez Franco R, Montoya A, Faull P, Kramer H, Vannier JB, TRF1 averts chromatin remodelling, recombination and replication dependent-break induced replication at mouse telomeres. Elife, 9():(2020) [pubmed]

curator keyword: Biological

submitter keyword: Mus Musculus, Embryonic Fibroblasts, Teleomere, Shelterin complex, LC-MSMS

Jean-Baptiste Vannier
contact affiliation	Medical Research Council - London Institute of Medical Sciences, Telomere Replication and Stability Group, United Kingdom.
contact email	j.vannier@lms.mrc.ac.uk
lab head
Alex Montoya
contact affiliation	Medical Research Council - London Institute of Medical Sciences
contact email	alex.montoya@lms.mrc.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD017022

PRIDE project URI

• PRIDE
  1. PXD017022
     1. Label: PRIDE project
     2. Name: TRF1 averts chromatin remodelling, recombination and replication dependent-Break Induced Replication at mouse telomeres