PXD016940-1

PXD016940 is an original dataset announced via ProteomeXchange.

Title	TMTpro16plex Isobaric Labeling Reagents: Attaining New Sample Multiplexing Heights and New Cellular Proteome Depths in Quantitative Proteomics
Description	Isobaric labeling empowers proteome-wide expression measurements simultaneously across multiple samples. Here, an expanded set of 16 isobaric reagents based on a proline backbone (TMTpro) is presented. These reagents have similar characteristics to existing TMT reagents but with increased fragmentation efficiency and signal. In a proteome-scale example dataset, we compared eight common cell lines with and without Torin1 treatment with three replicates, quantifying more than 8,800 proteins (mean of 7.5 peptides/protein) per replicate with an analysis time of only 67 min per proteome examined. Finally, we modified the powerful thermal stability assay to examine proteome-wide melting shifts after treatment with DMSO, 1 or 20 ������M staurosporine with five replicates. This new assay identified and dose-stratified the staurosporine binding to 228 cellular kinases in just one, 18-hr experiment. TMTpro reagents allow unprecedented complexity in experimental designs���������all with basically no missing values across the 16 samples and no loss in quantitative integrity.
HostingRepository	PRIDE
AnnounceDate	2020-03-19
AnnouncementXML	Submission_2020-03-19_16:35:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiaming Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-05 22:01:41	ID requested
⏵ 1	2020-03-19 16:35:38	announced
2	2020-03-25 07:19:27	announced	2020-03-25: Updated publication reference for PubMed record(s): 32203386.

Dataset with its publication pending

curator keyword: Technical

submitter keyword: Human, cell lines, SPS-MS3, TMTpro, proteomics

Steven P. Gygi
contact affiliation	Department of Cell Biology, Harvard Medical School, Boston, MA, USA
contact email	Steven_Gygi@hms.harvard.edu
lab head
Jiaming Li
contact affiliation	Harvard Medical School
contact email	jiaming_li@hms.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016940
     1. Label: PRIDE project
     2. Name: TMTpro16plex Isobaric Labeling Reagents: Attaining New Sample Multiplexing Heights and New Cellular Proteome Depths in Quantitative Proteomics