PXD016896

PXD016896 is an original dataset announced via ProteomeXchange.

Title	Screening and identification of ovulation-inducing factors in Bactrian camel seminal plasma
Description	Camelidae are inducing ovulators is tightly regulated by multiple factors, and understanding the biological mechanisms underlying follicular development, hormone secretion and ovulation requires investigation of the potential molecular pathways. However, little is known about the pathways of these factors in the camel. To identify candidate biomarkers of inducing ovulators, we performed a comprehensive proteomic and molecular biology analysis of the ovary from different groups. We identified 5,075 ovarian expressed proteins, of which 404 were differentially expressed (260 up-regulated, 144 down-regulated) in samples from treated versus control camels. Gene ontology annotation identified potential functions of the differentially expressed proteins (DEPs). We validated the differential expression for a subset of these proteins using qPCR, western blotting and immunofluorescence staining. Five DEPs (CAMK2G, FST, NR5A1, PAK1 and PRL) were involved in neurochemical signal transduction, endocrine and reproductive hormones regulatory processes. KEGG analysis indicated the involvement of several pathways, such as calcium, cAMP, gonadotropin-releasing hormone, MAPK, and neuroactive ligand-receptor signaling pathways, further suggesting that the induced ovulation process depends on the hypothalamic-pituitary-ovarian axis. We conclude that the profiling of ovarian proteomes, followed by the measurement of selected proteins using more targeted methods, offers a promising approach for studying the mechanisms of induced ovulation.
HostingRepository	iProX
AnnounceDate	2019-12-23
AnnouncementXML	Submission_2023-03-01_19:24:34.172.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qi Wang
SpeciesList	scientific name: Camelus bactrianus; NCBI TaxID: 9837;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-22 18:42:08	ID requested
1	2019-12-22 18:43:28	announced
⏵ 2	2023-03-01 19:24:35	announced	2023-03-02: Update publication information.

Wang Q, Zhang Q, Li Y, Zhao X, Zhang Y, Screening and Identification of Differential Ovarian Proteins before and after Induced Ovulation via Seminal Plasma in Bactrian Camels. Animals (Basel), 11(12):(2021) [pubmed]

submitter keyword: iTRAQ, Induced ovulation, Ovary, Bactrian camel

Xing Xu
contact affiliation	Gansu Agricultural University
contact email	zhaoxx@gsau.edu.cn
lab head
Qi Wang
contact affiliation	Gansu Agrucultural University
contact email	forever45214125@126.com
dataset submitter

iProX dataset URI