PXD016887-1

PXD016887 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics analysis of Angiostrongylus vasorum-induced alterations in dog serum
Description	Blood contains hundreds of proteins, reflecting ongoing cellular processes and immune reactions. Angiostrongylus vasorum infection is associated with a perturbed blood protein profile in dogs. However, the literature currently available lacks the necessary depth of analysis in order to resolve the observed pathologies in A. vasorum infections, including bleeding disorders. Using sera from 8 experimentally-infected dogs (i) before infection with A. vasorum, (ii) 34 days post-infection (p.i.; immature infection), and (iii) 75 days p.i. (mature patent infection), serum proteins were measured using liquid chromatography, tandem mass spectrometry (LC-MS/MS). For 2 dogs, serum was analyzed at days 104 and 230 p.i. additionally. A data-independent acquisition workflow was employed in order to generate quantitative data. Following computational analysis, we identified 139 up- and down-regulated proteins following infection (log2 ratio cutoff ≥ 1.0; q-value ≤ 0.05). Differences in serum profiles were most pronounced at day 75 p.i. compared to before infection. Among up-regulated proteins, chitinase 3, several saposin-like proteins, and heat shock proteins were found greatly increased (log2 fold-changes ≥ 5). Levels of pulmonary surfactant protein B were elevated on day 34 p.i. already, in the prepatent phase. Pathway enrichment revealed that complement (especially the lectin pathway) and coagulation cascades as significantly affected upon analysis of down-regulated proteins. Among them were mannan-binding lectin serine peptidases, ficolin, and coagulation factors. These results reflect the ongoing immune response and stress imposed to the lungs by the parasite. In addition, they bring new elements towards understanding the coagulopathies observed in some A. vasorum-infected dogs.
HostingRepository	PanoramaPublic
AnnounceDate	2021-01-12
AnnouncementXML	Submission_2021-01-12_08:10:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tobias Kockmann
SpeciesList	scientific name: Canis lupus familiaris; NCBI TaxID: 9615;
ModificationList	Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-20 08:47:13	ID requested
⏵ 1	2021-01-12 08:10:45	announced
2	2021-01-24 20:28:15	announced	: Added PubMed ID

Tritten L, Gillis-Germitsch N, Kockmann T, Schnyder M. Quantitative proteomics analysis of Angiostrongylus vasorum-induced alterations in dog serum sheds light on the pathogenesis of canine angiostrongylosis. Sci Rep [Internet]. 2021;11(1):283. Available from: https://doi.org/10.1038/s41598-020-79459-9

submitter keyword: Angiostrongylus vasorum

Manuela Schnyder
contact affiliation	University of Zurich (UZH)
contact email	manuela.schnyder@uzh.ch
lab head
Tobias Kockmann
contact affiliation	Functional Genomics Center Zurich
contact email	tobias.kockmann@fgcz.ethz.ch
dataset submitter

Panorama Public dataset URI