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PXD016881-2

PXD016881 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative proteomics analysis of Angiostrongylus vasorum-induced alterations in dog serum
DescriptionBlood contains hundreds of proteins, reflecting ongoing cellular processes and immune reactions. Angiostrongylus vasorum infection is associated with a perturbed blood protein profile in dogs. However, the literature currently available lacks the necessary depth of analysis in order to resolve the observed pathologies in A. vasorum infections, including bleeding disorders. Using sera from 8 experimentally-infected dogs (i) before infection with A. vasorum, (ii) 34 days post-infection (p.i.; immature infection), and (iii) 75 days p.i. (mature patent infection), serum proteins were measured using liquid chromatography, tandem mass spectrometry (LC-MS/MS). For 2 dogs, serum was analyzed at days 104 and 230 p.i. additionally. A data-independent acquisition workflow was employed in order to generate quantitative data. Following computational analysis, we identified 139 up- and down-regulated proteins following infection (log2 ratio cutoff ≥ 1.0; q-value ≤ 0.05). Differences in serum profiles were most pronounced at day 75 p.i. compared to before infection. Among up-regulated proteins, chitinase 3, several saposin-like proteins, and heat shock proteins were found greatly increased (log2 fold-changes ≥ 5). Levels of pulmonary surfactant protein B were elevated on day 34 p.i. already, in the prepatent phase. Pathway enrichment revealed that complement (especially the lectin pathway) and coagulation cascades as significantly affected upon analysis of down-regulated proteins. Among them were mannan-binding lectin serine peptidases, ficolin, and coagulation factors. These results reflect the ongoing immune response and stress imposed to the lungs by the parasite. In addition, they bring new elements towards understanding the coagulopathies observed in some A. vasorum-infected dogs.
HostingRepositoryPRIDE
AnnounceDate2021-01-12
AnnouncementXMLSubmission_2021-01-14_00:38:40.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTobias Kockmann
SpeciesList scientific name: Canis lupus familiaris; NCBI TaxID: 9615;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-12-20 06:46:06ID requested
12021-01-12 08:13:16announced
22021-01-14 00:38:41announced2021-01-14: Updated publication reference for PubMed record(s): 33431914.
Publication List
Tritten L, Gillis-Germitsch N, Kockmann T, Schnyder M, Quantitative proteomics analysis of Angiostrongylus vasorum-induced alterations in dog serum sheds light on the pathogenesis of canine angiostrongylosis. Sci Rep, 11(1):283(2021) [pubmed]
Keyword List
submitter keyword: dog, Angiostrongylus vasorum
Contact List
Manuela Schnyder
contact affiliationInstitute of Parasitology, Vetsuisse Faculty (VET), University of Zurich (UZH)
contact emailmanuela.schnyder@uzh.ch
lab head
Tobias Kockmann
contact affiliationFunctional Genomics Center Zurich
contact emailtobias.kockmann@fgcz.ethz.ch
dataset submitter
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PRIDE project URI
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