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PXD016826-1

PXD016826 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuman POT1 Prevents Severe Telomere Damage Instability Induced by Homology Directed DNA Repair
DescriptionThe evolutionarily conserved POT1 protein binds the single stranded G-rich telomeric DNA and has been implicated in telomeric DNA maintenance and the suppression of DNA damage checkpoint signaling. Here, we explore human POT1 function through genetics and proteomics discovering that the complete absence of POT1 leads to severe telomere maintenance defects that had not been anticipated from previous depletion studies. We determine the telomeric proteome upon POT1-loss by implementing an improved telomeric chromatin isolation protocol. Using quantitative proteomics by tandem mass tags (TMT) we identified a large set of proteins involved in nucleic acid metabolism that engage with telomeres upon loss of POT1. Inactivation of the homology directed repair machinery suppresses POT1-loss mediated telomeric DNA defects. Our results unravel as major function of human POT1 the suppression of telomere instability induced by homology directed repair.
HostingRepositoryPRIDE
AnnounceDate2020-10-21
AnnouncementXMLSubmission_2020-10-21_00:44:20.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterManfredo Quadroni
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-12-19 01:41:34ID requested
12020-10-21 00:44:22announced
Publication List
Glousker G, Briod AS, Quadroni M, Lingner J, Human shelterin protein POT1 prevents severe telomere instability induced by homology-directed DNA repair. EMBO J, 39(23):e104500(2020) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Telomeres, Homology-directed DNA repair, affinity purification, mass spectrometry, tandem mass tags
Contact List
Joachim Lingner
contact affiliationSwiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland
contact emailjoachim.lingner@epfl.ch
lab head
Manfredo Quadroni
contact affiliationUniversity of Lausanne
contact emailmanfredo.quadroni@unil.ch
dataset submitter
Full Dataset Link List
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PRIDE project URI
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