PXD016826-1

PXD016826 is an original dataset announced via ProteomeXchange.

Title	Human POT1 Prevents Severe Telomere Damage Instability Induced by Homology Directed DNA Repair
Description	The evolutionarily conserved POT1 protein binds the single stranded G-rich telomeric DNA and has been implicated in telomeric DNA maintenance and the suppression of DNA damage checkpoint signaling. Here, we explore human POT1 function through genetics and proteomics discovering that the complete absence of POT1 leads to severe telomere maintenance defects that had not been anticipated from previous depletion studies. We determine the telomeric proteome upon POT1-loss by implementing an improved telomeric chromatin isolation protocol. Using quantitative proteomics by tandem mass tags (TMT) we identified a large set of proteins involved in nucleic acid metabolism that engage with telomeres upon loss of POT1. Inactivation of the homology directed repair machinery suppresses POT1-loss mediated telomeric DNA defects. Our results unravel as major function of human POT1 the suppression of telomere instability induced by homology directed repair.
HostingRepository	PRIDE
AnnounceDate	2020-10-21
AnnouncementXML	Submission_2020-10-21_00:44:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manfredo Quadroni
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-19 01:41:34	ID requested
⏵ 1	2020-10-21 00:44:22	announced

Glousker G, Briod AS, Quadroni M, Lingner J, Human shelterin protein POT1 prevents severe telomere instability induced by homology-directed DNA repair. EMBO J, 39(23):e104500(2020) [pubmed]

curator keyword: Biological

submitter keyword: Telomeres, Homology-directed DNA repair, affinity purification, mass spectrometry, tandem mass tags

Joachim Lingner
contact affiliation	Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland
contact email	joachim.lingner@epfl.ch
lab head
Manfredo Quadroni
contact affiliation	University of Lausanne
contact email	manfredo.quadroni@unil.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD016826

PRIDE project URI

• PRIDE
  1. PXD016826
     1. Label: PRIDE project
     2. Name: Human POT1 Prevents Severe Telomere Damage Instability Induced by Homology Directed DNA Repair