PXD016815-1

PXD016815 is an original dataset announced via ProteomeXchange.

Title	Reactive oxygen species (ROS) production triggers unconventional secretion of antioxidant enzymes
Description	Nutrient deprivation triggers the release of signal-sequence-lacking antioxidant superoxide dismutase 1 (SOD1). We now report that secreted SOD1 is functionally active and accompanied by export of other antioxidant enzymes such as thioredoxins, (Trx1 and Trx2) and peroxiredoxin Ahp1. Our data reveal that starvation increases mitochondrial activity, which generates higher, but non-toxic, levels of reactive oxygen species (ROS). Inhibiting mitochondrial activity or ROS prevents antioxidants secretion. We suggest that in response to ROS production that can permeate the plasma membrane, the cells respond by secreting antioxidants to prevent ROS-mediated damage in the extracellular space. These findings provide an understanding of why cells secrete signal sequence lacking antioxidant enzymes in response to starvation.
HostingRepository	PRIDE
AnnounceDate	2020-03-26
AnnouncementXML	Submission_2020-03-26_03:10:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eduard Sabid��
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	N-acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-18 01:26:32	ID requested
⏵ 1	2020-03-26 03:10:35	announced
2	2020-06-12 02:52:29	announced	2020-06-12: Updated publication reference for PubMed record(s): 32328640.

Dataset with its publication pending

submitter keyword: proteomics, ROS, yeast

Amy Curwin
contact affiliation	Centre for Genomic Regulation, The Barcelona Institute of Science and Technology, 08003 Barcelona, Spain
contact email	amy.curwin@crg.eu
lab head
Eduard Sabid��
contact affiliation	Centre de Regulaci�� Gen��mica
contact email	eduard.sabido@crg.cat
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD016815

PRIDE project URI

• PRIDE
  1. PXD016815
     1. Label: PRIDE project
     2. Name: Reactive oxygen species (ROS) production triggers unconventional secretion of antioxidant enzymes