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PXD016815

PXD016815 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleReactive oxygen species (ROS) production triggers unconventional secretion of antioxidant enzymes
DescriptionNutrient deprivation triggers the release of signal-sequence-lacking antioxidant superoxide dismutase 1 (SOD1). We now report that secreted SOD1 is functionally active and accompanied by export of other antioxidant enzymes such as thioredoxins, (Trx1 and Trx2) and peroxiredoxin Ahp1. Our data reveal that starvation increases mitochondrial activity, which generates higher, but non-toxic, levels of reactive oxygen species (ROS). Inhibiting mitochondrial activity or ROS prevents antioxidants secretion. We suggest that in response to ROS production that can permeate the plasma membrane, the cells respond by secreting antioxidants to prevent ROS-mediated damage in the extracellular space. These findings provide an understanding of why cells secrete signal sequence lacking antioxidant enzymes in response to starvation.
HostingRepositoryPRIDE
AnnounceDate2020-03-26
AnnouncementXMLSubmission_2020-03-26_03:10:34.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEduard Sabid��
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListN-acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-12-18 01:26:32ID requested
12020-03-26 03:10:35announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: proteomics, ROS, yeast
Contact List
Amy Curwin
contact affiliationCentre for Genomic Regulation, The Barcelona Institute of Science and Technology, 08003 Barcelona, Spain
contact emailamy.curwin@crg.eu
lab head
Eduard Sabid��
contact affiliationCentre de Regulaci�� Gen��mica
contact emaileduard.sabido@crg.cat
dataset submitter
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PRIDE project URI
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