PXD016747 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomics of Giardia exposures to HKMT inhibitors |
Description | We explored differential protein expression (DEPs) during sublethal exposures of HKMT inhibitors (BIX-01294 = 10uM (B), Chaetocin = 20uM (C)) in log-phase (growth; TY) and encystation (differentiation; EC) at 8 and 24 hours relative to DMSO controls (D). In total, 18,527 peptides from 1,981 proteins in log-phase, and 19,852 peptides from 2,097 proteins were analysed in encysting cultures. In total, 56 proteins were up- and 36 down-regulated in log-phase trophozoites and 32 up- and 12 down during encystation upon BIX-01294-treatment relative to the DMSO controls. Following Chaetocin-treatment, 195 proteins were up- and 121 down-regulated in log-phase trophozoites, and 63 up- and 12 down during encystation. We have presented results by cell-phase given positive correlations were driven by culture condition and not between either Chaetocin or BIX-01294 inhibitor). |
HostingRepository | PRIDE |
AnnounceDate | 2020-08-04 |
AnnouncementXML | Submission_2020-08-03_22:17:16.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Samantha Emery |
SpeciesList | scientific name: Giardia lamblia ATCC 50803; NCBI TaxID: 184922; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-12-13 08:24:17 | ID requested | |
⏵ 1 | 2020-08-03 22:17:17 | announced | |
Publication List
Emery-Corbin SJ, Hamey JJ, Ansell BRE, Balan B, Tichkule S, Stroehlein AJ, Cooper C, McInerney BV, Hediyeh-Zadeh S, Vuong D, Crombie A, Lacey E, Davis MJ, Wilkins MR, Bahlo M, Sv, รค, rd SG, Gasser RB, Jex AR, Eukaryote-Conserved Methylarginine Is Absent in Diplomonads and Functionally Compensated in Giardia. Mol Biol Evol, 37(12):3525-3549(2020) [pubmed] |
Keyword List
submitter keyword: Giardia, protein methylation, HKMT inhibitor, BIX-01294, Chaetocin |
Contact List
Aaron Jex |
contact affiliation | Population Health and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, Australia Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Melbourne, VIC, Australia |
contact email | jex.a@wehi.edu.au |
lab head | |
Samantha Emery |
contact affiliation | Population Health and Immunity, Walter and Eliza Hall Institute of Medical Research |
contact email | emery.s@wehi.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016747
- Label: PRIDE project
- Name: Quantitative proteomics of Giardia exposures to HKMT inhibitors