PXD016747-1

PXD016747 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics of Giardia exposures to HKMT inhibitors
Description	We explored differential protein expression (DEPs) during sublethal exposures of HKMT inhibitors (BIX-01294 = 10uM (B), Chaetocin = 20uM (C)) in log-phase (growth; TY) and encystation (differentiation; EC) at 8 and 24 hours relative to DMSO controls (D). In total, 18,527 peptides from 1,981 proteins in log-phase, and 19,852 peptides from 2,097 proteins were analysed in encysting cultures. In total, 56 proteins were up- and 36 down-regulated in log-phase trophozoites and 32 up- and 12 down during encystation upon BIX-01294-treatment relative to the DMSO controls. Following Chaetocin-treatment, 195 proteins were up- and 121 down-regulated in log-phase trophozoites, and 63 up- and 12 down during encystation. We have presented results by cell-phase given positive correlations were driven by culture condition and not between either Chaetocin or BIX-01294 inhibitor).
HostingRepository	PRIDE
AnnounceDate	2020-08-04
AnnouncementXML	Submission_2020-08-03_22:17:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Samantha Emery
SpeciesList	scientific name: Giardia lamblia ATCC 50803; NCBI TaxID: 184922;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-13 08:24:17	ID requested
⏵ 1	2020-08-03 22:17:17	announced

Emery-Corbin SJ, Hamey JJ, Ansell BRE, Balan B, Tichkule S, Stroehlein AJ, Cooper C, McInerney BV, Hediyeh-Zadeh S, Vuong D, Crombie A, Lacey E, Davis MJ, Wilkins MR, Bahlo M, Sv, ä, rd SG, Gasser RB, Jex AR, Eukaryote-Conserved Methylarginine Is Absent in Diplomonads and Functionally Compensated in Giardia. Mol Biol Evol, 37(12):3525-3549(2020) [pubmed]

submitter keyword: Giardia, protein methylation, HKMT inhibitor, BIX-01294, Chaetocin

Aaron Jex
contact affiliation	Population Health and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, Australia Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Melbourne, VIC, Australia
contact email	jex.a@wehi.edu.au
lab head
Samantha Emery
contact affiliation	Population Health and Immunity, Walter and Eliza Hall Institute of Medical Research
contact email	emery.s@wehi.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD016747

PRIDE project URI

• PRIDE
  1. PXD016747
     1. Label: PRIDE project
     2. Name: Quantitative proteomics of Giardia exposures to HKMT inhibitors