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PXD016747

PXD016747 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative proteomics of Giardia exposures to HKMT inhibitors
DescriptionWe explored differential protein expression (DEPs) during sublethal exposures of HKMT inhibitors (BIX-01294 = 10uM (B), Chaetocin = 20uM (C)) in log-phase (growth; TY) and encystation (differentiation; EC) at 8 and 24 hours relative to DMSO controls (D). In total, 18,527 peptides from 1,981 proteins in log-phase, and 19,852 peptides from 2,097 proteins were analysed in encysting cultures. In total, 56 proteins were up- and 36 down-regulated in log-phase trophozoites and 32 up- and 12 down during encystation upon BIX-01294-treatment relative to the DMSO controls. Following Chaetocin-treatment, 195 proteins were up- and 121 down-regulated in log-phase trophozoites, and 63 up- and 12 down during encystation. We have presented results by cell-phase given positive correlations were driven by culture condition and not between either Chaetocin or BIX-01294 inhibitor).
HostingRepositoryPRIDE
AnnounceDate2020-08-04
AnnouncementXMLSubmission_2020-08-03_22:17:16.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSamantha Emery
SpeciesList scientific name: Giardia lamblia ATCC 50803; NCBI TaxID: 184922;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-12-13 08:24:17ID requested
12020-08-03 22:17:17announced
Publication List
Emery-Corbin SJ, Hamey JJ, Ansell BRE, Balan B, Tichkule S, Stroehlein AJ, Cooper C, McInerney BV, Zadeh SH, Vuong D, Crombie A, Lacey E, Davis MJ, Wilkins MR, Bahlo M, Svärd SG, Gasser RB, Jex AR, Eukaryote-conserved methylarginine is absent in diplomonads and functionally compensated in Giardia. Mol Biol Evol, ():(2020) [pubmed]
Keyword List
submitter keyword: Giardia, protein methylation, HKMT inhibitor, BIX-01294, Chaetocin
Contact List
Aaron Jex
contact affiliationPopulation Health and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, Australia Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Melbourne, VIC, Australia
contact emailjex.a@wehi.edu.au
lab head
Samantha Emery
contact affiliationPopulation Health and Immunity, Walter and Eliza Hall Institute of Medical Research
contact emailemery.s@wehi.edu.au
dataset submitter
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