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PXD016679 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe bovine cumulus proteome of cumulus oocyte complexes that successfully matured or failed to mature after maturation in-vitro and in-vivo
DescriptionThe oocyte forms a complex with their somatic cumulus cells within the follicle throughout the preovulatory maturation steps. Cumulus cells support their oocyte not only through mechanical protection but also with a close bidirectional exchange of metabolites. Analysis of the oocytes cumulus gives the opportunity to explore non-invasively oocytal well-being and quality. In vitro maturation (IVM) is the first rate-limiting step in in vitro embryo production. Analysis of protein expression in cumulus cells around this critical step helps to explore the impact of maturation conditions and to examine an influence on maturational competence of the oocyte. The goal of this study was the comparison of the cumulus proteome of oocytes with and without maturational competence matured under in vivo and in vitro conditions. Therefore twenty cumulus samples corresponding to single oocytes were analysed. Half of the samples were matured in vivo and the other half in vitro. For each maturation group, cumulus from oocytes matured successfully (SM; n=5) and failed to mature (FM; n=5) were analysed.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJasmin Walter
SpeciesList scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-12-10 07:10:59ID requested
12020-06-29 23:14:25announced
Publication List
Walter J, Monthoux C, Fortes C, Grossmann J, Roschitzki B, Meili T, Riond B, Hofmann-Lehmann R, Naegeli H, Bleul U, The bovine cumulus proteome is influenced by maturation condition and maturational competence of the oocyte. Sci Rep, 10(1):9880(2020) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Oocyte, Cumulus, Proteomics
Contact List
Ulrich Bleul
contact affiliationClinic of Reproductive Medicine, Vetsuisse Faculty, University of Zurich
contact emailubleul@vetclinics.uzh.ch
lab head
Jasmin Walter
contact affiliationClinic of Reproductive Medicine, Vetsuisse Faculty, University of Zurich
contact emailjwalter@vetclinics.uzh.ch
dataset submitter
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