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PXD016656-1

PXD016656 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEmbryo-like features in developing Bacillus subtilis biofilms
DescriptionCorrespondence between evolution and development has been discussed for more than two centuries. Recent work reveals that phylogeny-ontogeny correlations are indeed present in developmental transcriptomes of eukaryotic clades with complex multicellularity. Nevertheless, it has been largely ignored that the pervasive presence of phylogeny-ontogeny correlations is likely a hallmark of development in eukaryotes. This perspective opens a possibility to look for similar parallelisms in biological settings where developmental logic and multicellular complexity are more obscure. For instance, it has been increasingly recognized that multicellular behavior underlies biofilm formation in bacteria. However, it remains unclear whether bacterial biofilm growth shares some basic principles with development in complex eukaryotes. Here we show that ontogeny of growing Bacillus subtilis biofilms recapitulates phylogeny at the expression level. Using finely resolved transcriptome and proteome profiles, we found that biofilm ontogeny correlates with the evolutionary measures. Early-stage biofilms expressed older and more conserved genes, while later-stage biofilms progressively used evolutionary younger and more diverged genes. Molecular and morphological signatures also revealed that biofilm growth is highly regulated and organized into discrete ontogenetic stages, similar to those of eukaryotic embryos. In conjunction this suggests that biofilm formation in Bacillus is a bona fide developmental process comparable to organismal development in animals, plants and fungi. Given that most cells on Earth reside in the form of biofilms and that biofilms represent the oldest known fossils, we anticipate that the widely-adopted vision of the first life as a single-cell and free-living organism needs rethinking.
HostingRepositoryPRIDE
AnnounceDate2020-10-07
AnnouncementXMLSubmission_2020-10-06_22:50:42.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterVaishnavi Ravikumar
SpeciesList scientific name: Bacillus subtilis subsp. subtilis str. NCIB 3610; NCBI TaxID: 535026;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-12-09 01:37:34ID requested
12020-10-06 22:50:43announced
Publication List
Futo M, Opašić L, Koska S, Čorak N, Široki T, Ravikumar V, Thorsell A, Lenuzzi M, Kifer D, Domazet-Lošo M, Vlahoviček K, Mijakovic I, Domazet-Lošo T, Embryo-Like Features in Developing Bacillus subtilis Biofilms. Mol Biol Evol, 38(1):31-47(2021) [pubmed]
Keyword List
submitter keyword: Bacillus subtilis, biofilm, phylostratigraphy, proteomics, transcriptomics
Contact List
Tomislav Domazet-Lošo
contact affiliationLaboratory of Evolutionary Genetics, Ruđer Bošković Institute, Bijenička cesta 54, HR-10000 Zagreb, Croatia.
contact emailtdomazet@irb.hr
lab head
Vaishnavi Ravikumar
contact affiliationTechnical University of Denmark
contact emailvairav@biosustain.dtu.dk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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