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PXD016633 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMouse Th17 T cells, IL-23 stimulated
DescriptionInterleukin 23 (IL-23) triggers pathogenic features in pro-inflammatory, IL-17-secreting T cells (Th17 and Tγδ17) that play a key role in the development of inflammatory diseases. However, the IL-23 signaling cascade remains largely undefined. Here we used quantitative phosphoproteomics to characterize IL-23 signaling in primary murine Th17 cells. We quantified 6,888 phosphorylation sites in Th17 cells, and found 168 phosphorylations regulated upom IL-23 stimulation. IL-23 increased the phosphorylation of the myosin regulatory light chain (RLC), an actomyosin contractibility marker, in Th17 and Tγδ cells. IL-23-induced RLC phosphorylation required JAK2 and ROCK catalytic activity, and the study of the IL-23/ROCK axis revealed an unexpected role of IL-23 in the migration of Tγδ17 and Th17 cells. Moreover, pharmacological inhibition of ROCK reduced Tγδ17 recruitment to inflamed skin upon challenge with inflammatory agent Imiquimod. This work: i) provides new insights into phosphorylation networks that control Th17 cells, ii) widely expands the current knowledge on IL-23 signaling, and iii) contributes to the increasing list of immune cells subsets characterized by global phosphoproteomic approaches.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMaria Navarro
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-12-06 05:22:06ID requested
12020-03-25 07:13:37announced
Publication List
Álvarez-Salamero C, Castillo-González R, Pastor-Fernández G, Mariblanca IR, Pino J, Cibrian D, Navarro MN, IL-23 signaling regulation of pro-inflammatory T-cell migration uncovered by phosphoproteomics. PLoS Biol, 18(3):e3000646(2020) [pubmed]
Keyword List
submitter keyword: Mouse, T lymphocyte, Th17, IL-23
Contact List
Maria N. Navarro
contact affiliationMaría N. Navarro Interactions With The Environment Program. Immune System Development and Function Unit. Centro de Biología Molecular "Severo Ochoa" Lab. 126 C/Nicolás Cabrera, 1. 28049 Madrid. Spain Tlf.: (+34) 911964453 email: marian.navarro@cbm.csic.es
contact emailmarian.navarro@cbm.csic.es
lab head
Maria Navarro
contact affiliationCentro de Biología Molecular "Severo Ochoa"
contact emailmarian.navarro@cbm.csic.es
dataset submitter
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