PXD016633

PXD016633 is an original dataset announced via ProteomeXchange.

Title	Mouse Th17 T cells, IL-23 stimulated
Description	Interleukin 23 (IL-23) triggers pathogenic features in pro-inflammatory, IL-17-secreting T cells (Th17 and Tγδ17) that play a key role in the development of inflammatory diseases. However, the IL-23 signaling cascade remains largely undefined. Here we used quantitative phosphoproteomics to characterize IL-23 signaling in primary murine Th17 cells. We quantified 6,888 phosphorylation sites in Th17 cells, and found 168 phosphorylations regulated upom IL-23 stimulation. IL-23 increased the phosphorylation of the myosin regulatory light chain (RLC), an actomyosin contractibility marker, in Th17 and Tγδ cells. IL-23-induced RLC phosphorylation required JAK2 and ROCK catalytic activity, and the study of the IL-23/ROCK axis revealed an unexpected role of IL-23 in the migration of Tγδ17 and Th17 cells. Moreover, pharmacological inhibition of ROCK reduced Tγδ17 recruitment to inflamed skin upon challenge with inflammatory agent Imiquimod. This work: i) provides new insights into phosphorylation networks that control Th17 cells, ii) widely expands the current knowledge on IL-23 signaling, and iii) contributes to the increasing list of immune cells subsets characterized by global phosphoproteomic approaches.
HostingRepository	PRIDE
AnnounceDate	2020-03-25
AnnouncementXML	Submission_2020-03-25_07:13:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maria Navarro
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-06 05:22:06	ID requested
⏵ 1	2020-03-25 07:13:37	announced

Á, lvarez-Salamero C, Castillo-Gonz, á, lez R, Pastor-Fern, á, ndez G, Mariblanca IR, Pino J, Cibrian D, Navarro MN, IL-23 signaling regulation of pro-inflammatory T-cell migration uncovered by phosphoproteomics. PLoS Biol, 18(3):e3000646(2020) [pubmed]

submitter keyword: Mouse, T lymphocyte, Th17, IL-23

Maria N. Navarro
contact affiliation	María N. Navarro Interactions With The Environment Program. Immune System Development and Function Unit. Centro de Biología Molecular "Severo Ochoa" Lab. 126 C/Nicolás Cabrera, 1. 28049 Madrid. Spain Tlf.: (+34) 911964453 email: marian.navarro@cbm.csic.es
contact email	marian.navarro@cbm.csic.es
lab head
Maria Navarro
contact affiliation	Centro de Biología Molecular "Severo Ochoa"
contact email	marian.navarro@cbm.csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD016633

PRIDE project URI

• PRIDE
  1. PXD016633
     1. Label: PRIDE project
     2. Name: Mouse Th17 T cells, IL-23 stimulated