PXD016576 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Protease-resistant streptavidin for interaction proteomics |
Description | Streptavidin-mediated enrichment is a powerful strategy to identify biotinylated biomolecules and their interaction partners by mass spectrometry. Since contamination by streptavidin limits protein identification, we here present protease-resistant streptavidin beads (prS). Their application to different biotinylated biomolecules (DNA, protein and lipid) result in >100 streptavidin contamination and better coverage of both the target and its protein interactors without the need for sample pre-fractionation. |
HostingRepository | PRIDE |
AnnounceDate | 2020-05-27 |
AnnouncementXML | Submission_2020-05-26_16:13:35.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gianluca Sigismondo |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | biotinylated residue; 6x(13)C labeled residue; iodoacetamide derivatized residue |
Instrument | Q Exactive; Orbitrap Fusion; LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-12-04 03:32:48 | ID requested | |
⏵ 1 | 2020-05-26 16:13:36 | announced | |
Publication List
Rafiee MR, Sigismondo G, Kalxdorf M, F, ö, rster L, Br, ü, gger B, B, é, thune J, Krijgsveld J, Protease-resistant streptavidin for interaction proteomics. Mol Syst Biol, 16(5):e9370(2020) [pubmed] |
Keyword List
submitter keyword: streptavidin, biotin, mass spectrometry, affinity purification, BioID, membrane protein, DNA pull down, ChIP, ChIP SICAP, lipid binding protein |
Contact List
Jeroen Krijgsveld |
contact affiliation | Division of Proteomics of Stem Cells and Cancer |
contact email | j.krijgsveld@dkfz.de |
lab head | |
Gianluca Sigismondo |
contact affiliation | DKFZ |
contact email | gianluca.sigismondo@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD016576 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016576
- Label: PRIDE project
- Name: Protease-resistant streptavidin for interaction proteomics