PXD016576

PXD016576 is an original dataset announced via ProteomeXchange.

Title	Protease-resistant streptavidin for interaction proteomics
Description	Streptavidin-mediated enrichment is a powerful strategy to identify biotinylated biomolecules and their interaction partners by mass spectrometry. Since contamination by streptavidin limits protein identification, we here present protease-resistant streptavidin beads (prS). Their application to different biotinylated biomolecules (DNA, protein and lipid) result in >100 streptavidin contamination and better coverage of both the target and its protein interactors without the need for sample pre-fractionation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:05:51.664.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gianluca Sigismondo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue; 6x(13)C labeled residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion; Q Exactive; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-04 03:32:48	ID requested
1	2020-05-26 16:13:36	announced
⏵ 2	2024-10-22 05:05:56	announced	2024-10-22: Updated project metadata.

10.15252/msb.20199370;

Rafiee MR, Sigismondo G, Kalxdorf M, F, ö, rster L, Br, ü, gger B, B, é, thune J, Krijgsveld J, Protease-resistant streptavidin for interaction proteomics. Mol Syst Biol, 16(5):e9370(2020) [pubmed]

submitter keyword: streptavidin, affinity purification, mass spectrometry, DNA pull down, ChIP SICAP, lipid binding protein, BioID, membrane protein, ChIP, biotin

Jeroen Krijgsveld
contact affiliation	Division of Proteomics of Stem Cells and Cancer
contact email	j.krijgsveld@dkfz.de
lab head
Gianluca Sigismondo
contact affiliation	DKFZ
contact email	gianluca.sigismondo@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD016576

PRIDE project URI

• PRIDE
  1. PXD016576
     1. Label: PRIDE project
     2. Name: Protease-resistant streptavidin for interaction proteomics