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PXD016558-1

PXD016558 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEGFR inhibition triggers an adaptive response by co-opting antiviral signaling pathways in lung cancer
DescriptionHCC827 cells were treated with 0.1 microM erlotinib for 0, 2, 6, and 24 hours. Cell lysates were immunoprecipitated with TBK1 antibody. Antibody enriched protein samples were run on SDS-PAGE gels and submitted to UT Southwestern Proteomics Core Facility for Mass Spectrometry. Protein gel pieces were digested overnight with trypsin (Pierce) following reduction and alkylation with DTT and iodoacetamide (Sigma Aldrich. The samples then underwent solid-phase extraction cleanup with Oasis HLB microelution plates (Waters) and the resulting samples were analyzed by LC-MS-MS, using an Orbitrap Fusion Lumos mass spectrometer (Thermo Electron) coupled to an Ultimate 3000 RSLC-Nano liquid chromatography systems (Dionex).
HostingRepositoryMassIVE
AnnounceDate2019-12-03
AnnouncementXMLSubmission_2019-12-03_12:49:59.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMohammad
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-12-03 12:45:30ID requested
12019-12-03 12:50:00announced
Publication List
no publication
Keyword List
submitter keyword: EGFR inhibition, therapeutic resistance, Type I interferons, TBK1, IRF3,RIG-I, lung cancer, NSCLC, erlotinib, EGFR wild type, Anifrolumab
Contact List
Amyn A. Habib
contact affiliationUniversity of Texas Southwestern Medical Center, North Texas VA Healthcare System, Mail Code 151, 4500 South Lancaster Road, Dallas TX 75216
contact emailAmyn.Habib@UTSouthwestern.edu
lab head
Mohammad
contact affiliationUT Southwestern Medical center
contact emailmohammad.goodarzi@utsouthwestern.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084654/