PXD016539-1

PXD016539 is an original dataset announced via ProteomeXchange.

Title	PRM method for detection of both casein and whey milk allergens from baked food matrix
Description	Milk allergy is the most common food allergy presented in early childhood which in some cases may persist into adulthood as well. Proteins belonging to casein and whey fractions of milk trigger an allergic response in susceptible individuals. Milk is present as an ingredient in many foods and it can be also present as casein or whey enriched milk-derived ingredients. As whey proteins are more susceptible to thermal processing than caseins, conventional methods often posed a challenge in accurate detection of whey allergens, particularly from a processed complex food matrix. In this study we have developed a targeted mass spectrometry method to detect the presence of both casein and whey allergens from thermally processed foods. A pool of 19 candidate peptides representing four casein proteins and two whey proteins was identified using a discovery-driven target selection approach from various milk-derived ingredients. These target peptides were evaluated by parallel reaction monitoring of baked cookie samples containing known amounts of nonfat dry milk. The presence of milk could be detected from baked cookies incurred with NFDM at levels as low as 1 ppm using seven peptides representing α, β, and κ casein proteins and three peptides representing whey protein, β-lactoglobulin by this consensus PRM method.
HostingRepository	PanoramaPublic
AnnounceDate	2020-06-04
AnnouncementXML	Submission_2020-06-04_19:59:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bini Ramachandran
SpeciesList	scientific name: Bos taurus; NCBI TaxID: 9913;
ModificationList	Phospho; Carbamidomethyl; Oxidation
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-01 12:24:03	ID requested
⏵ 1	2020-06-04 19:59:01	announced

Ramachandran B, Yang CT, Downs ML, Parallel Reaction Monitoring Mass Spectrometry Method for Detection of Both Casein and Whey Milk Allergens from a Baked Food Matrix. J Proteome Res, 19(8):2964-2976(2020) [pubmed]

submitter keyword: Targeted mass spectrometry

PRM

Milk allergen detection

casein and whey proteins

baked food matrix

Melanie Downs
contact affiliation	University of Nebraska-Lincoln
contact email	mdowns2@unl.edu
lab head
Bini Ramachandran
contact affiliation	University of Nebraska-Lincoln
contact email	bramachandran2@unl.edu
dataset submitter

Panorama Public dataset URI