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PXD016539

PXD016539 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePRM method for detection of both casein and whey milk allergens from baked food matrix
DescriptionMilk allergy is the most common food allergy presented in early childhood which in some cases may persist into adulthood as well. Proteins belonging to casein and whey fractions of milk trigger an allergic response in susceptible individuals. Milk is present as an ingredient in many foods and it can be also present as casein or whey enriched milk-derived ingredients. As whey proteins are more susceptible to thermal processing than caseins, conventional methods often posed a challenge in accurate detection of whey allergens, particularly from a processed complex food matrix. In this study we have developed a targeted mass spectrometry method to detect the presence of both casein and whey allergens from thermally processed foods. A pool of 19 candidate peptides representing four casein proteins and two whey proteins was identified using a discovery-driven target selection approach from various milk-derived ingredients. These target peptides were evaluated by parallel reaction monitoring of baked cookie samples containing known amounts of nonfat dry milk. The presence of milk could be detected from baked cookies incurred with NFDM at levels as low as 1 ppm using seven peptides representing α, β, and κ casein proteins and three peptides representing whey protein, β-lactoglobulin by this consensus PRM method.
HostingRepositoryPanoramaPublic
AnnounceDate2020-06-04
AnnouncementXMLSubmission_2020-06-04_19:59:00.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterBini Ramachandran
SpeciesList scientific name: Bos taurus; NCBI TaxID: 9913;
ModificationListPhospho; Carbamidomethyl; Oxidation
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-12-01 12:24:03ID requested
12020-06-04 19:59:01announced
Publication List
Ramachandran B, Yang CT, Downs ML, Parallel Reaction Monitoring Mass Spectrometry Method for Detection of Both Casein and Whey Milk Allergens from a Baked Food Matrix. J Proteome Res, ():(2020) [pubmed]
Keyword List
submitter keyword: Targeted mass spectrometry
PRM
Milk allergen detection
casein and whey proteins
baked food matrix
Contact List
Melanie Downs
contact affiliationUniversity of Nebraska-Lincoln
contact emailmdowns2@unl.edu
lab head
Bini Ramachandran
contact affiliationUniversity of Nebraska-Lincoln
contact emailbramachandran2@unl.edu
dataset submitter
Full Dataset Link List
Panorama Public dataset URI