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PXD016331 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative Live Cell Imaging Reveals Differential Manipulation of Rab11A Transport Pathways by Influenza virus and RSV
DescriptionAssembly of infectious influenza A viruses (IAV) is a complex process involving transport from the nucleus to the plasma membrane. Rab11A containing recycling endosomes have been identified as a platform for intracellular transport of viral RNA (vRNA). Using high spatiotemporal resolution light-sheet microscopy (~1.4 volumes/s, 330 nm isotropic spatial resolution), we quantify Rab11A and vRNA movement in live cells during IAV infection. Here we report that IAV infection decreases speed and increases arrest of Rab11A. Unexpectedly, infection with RSV alters Rab11A motion in a manner opposite to IAV, suggesting that Rab11A is a common host component that is differentially manipulated by respiratory RNA viruses. Using two-color imaging we demonstrate co-transport between Rab11A and IAV vRNA in infected cells and provide direct evidence that vRNA-associated Rab11A have altered transport. The mechanism of altered Rab11A movement is likely related to a decrease in dynein motors bound to Rab11A vesicles during IAV infection.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterKatarzyna Kulej
SpeciesList scientific name: Influenza A virus (A/California/07/2009(H1N1)); NCBI TaxID: 641809; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListOxidation; Acetyl; Carbamidomethyl
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-11-19 01:48:00ID requested
12020-01-14 04:17:39announced
22020-01-17 06:14:50announced2020-01-17: Updated publication reference for PubMed record(s): 31911620.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: influenza virus, microtubules, Rab11A, endosomes, assembly, RNA, live-cell imaging, light-sheet microscopy, fluorescence microscopy, viral RNA, motor transport, vesicle tracking
Contact List
Benjamin A. Garcia
contact affiliationDepartment of Biochemistry and Biophysics Smilow Center for Translational Research Perelman School of Medicine University of Pennsylvania United States
contact emailbgarci@pennmedicine.upenn.edu
lab head
Katarzyna Kulej
contact affiliationChildren's Hospital of Philadelphia
contact emailkulej.katarzyna@gmail.com
dataset submitter
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