PXD016028-1
PXD016028 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Exoprotoemics of pseudomonas putida KT2440 outer membrane vesicles (OMV) and vesicle-free secretome (VSF) |
Description | Lignin is an abundant and recalcitrant component of plant cell walls. While lignin degradation in Nature is typically attributed to fungi, growing evidence suggests that bacteria can also modify this complex biopolymer. However, the spatiotemporal mechanisms for this modification remain unclear. Improved understanding of this biological and extracellular process would aid in our collective knowledge of both carbon cycling and advanced microbial strategies to valorize lignin. In this data set, we examined the exoproteome of Pseudomonas putida KT2440 grown with on lignin-rich media to characterize the secretion of abundant outer membrane vesicles (OMVs). Proteomic analysis revealed that the exoproteome is 20% more sub-compartmentalized into OMVs and OMV protein cargo are 52% more dynamic in lignin-rich media as compared to lignin-free media. |
HostingRepository | MassIVE |
AnnounceDate | 2020-03-12 |
AnnouncementXML | Submission_2020-03-12_12:12:56.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Paul Abraham |
SpeciesList | scientific name: Pseudomonas putida KT2440; NCBI TaxID: 160488; |
ModificationList | Oxidation; Carbamidomethyl |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-10-25 12:39:00 | ID requested | |
⏵ 1 | 2020-03-12 12:12:57 | announced |
Publication List
no publication |
Keyword List
submitter keyword: exoproteomics, proteomics, LC-MS/MS, outer membrane vesicles |
Contact List
Gregg Beckham | |
---|---|
contact affiliation | National Bioenergy Center, National Renewable Energy Laboratory |
contact email | gregg.beckham@nrel.gov |
lab head | |
Paul Abraham | |
contact affiliation | ORNL |
contact email | pav@ornl.gov |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084506/ |