PXD016028

PXD016028 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Exoprotoemics of pseudomonas putida KT2440 outer membrane vesicles (OMV) and vesicle-free secretome (VSF)
Description	Lignin is an abundant and recalcitrant component of plant cell walls. While lignin degradation in Nature is typically attributed to fungi, growing evidence suggests that bacteria can also modify this complex biopolymer. However, the spatiotemporal mechanisms for this modification remain unclear. Improved understanding of this biological and extracellular process would aid in our collective knowledge of both carbon cycling and advanced microbial strategies to valorize lignin. In this data set, we examined the exoproteome of Pseudomonas putida KT2440 grown with on lignin-rich media to characterize the secretion of abundant outer membrane vesicles (OMVs). Proteomic analysis revealed that the exoproteome is 20% more sub-compartmentalized into OMVs and OMV protein cargo are 52% more dynamic in lignin-rich media as compared to lignin-free media.
HostingRepository	MassIVE
AnnounceDate	2020-03-12
AnnouncementXML	Submission_2020-03-12_12:12:56.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paul Abraham
SpeciesList	scientific name: Pseudomonas putida KT2440; NCBI TaxID: 160488;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-25 12:39:00	ID requested
⏵ 1	2020-03-12 12:12:57	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: exoproteomics, proteomics, LC-MS/MS, outer membrane vesicles

submitter keyword: exoproteomics, proteomics, LC-MS/MS, outer membrane vesicles

Contact List

Gregg Beckham
contact affiliation	National Bioenergy Center, National Renewable Energy Laboratory
contact email	gregg.beckham@nrel.gov
lab head
Paul Abraham
contact affiliation	ORNL
contact email	pav@ornl.gov
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084506/