PXD015986-1

PXD015986 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS analysis of immunoprecipitation samples of Tetrahymena meiotic proteins Mcmd1 and Pamd1
Description	Recombinational repair of meiotic DNA double-strand breaks (DSBs) uses the homologous chromosome as a template, although the sister chromatid offers itself as a spatially more convenient substrate. In many organisms, this choice of the homolog rather than the sister is reinforced by the recombination protein Dmc1. We found that, in Tetrahymena, in the absence of two novel proteins, Mcmd1 and Pamd1, Dmc1 is misregulated. Hence, to find out potential interactions, we did LC-MS/MS analysis for Mcmd1 and Pamd1 immunoprecipitation samples.
HostingRepository	PRIDE
AnnounceDate	2019-11-19
AnnouncementXML	Submission_2019-11-19_00:16:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Gossenreiter
SpeciesList	scientific name: Tetrahymena thermophila; NCBI TaxID: 5911;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-23 05:51:06	ID requested
⏵ 1	2019-11-19 00:16:47	announced
2	2019-11-27 06:00:21	announced	2019-11-27: Updated project metadata.
3	2020-01-14 06:53:34	announced	2019-11-27: Updated project metadata. 2020-01-14: Updated publication reference for PubMed record(s): 31815942.

Dataset with its publication pending

submitter keyword: Tetrahymena thermophila, Meiosis, Immunoprecipitation, LC-MS/MS

Josef Loidl
contact affiliation	Department of Chromosome Biology, Max Perutz Labs, University of Vienna, 1030 Vienna, Austria
contact email	josef.loidl@univie.ac.at
lab head
Thomas Gossenreiter
contact affiliation	Mass Spectrometry Facility, Max F. Perutz Laboratories (MFPL), Vienna Biocenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna, Austria
contact email	thomas.gossenreiter@univie.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD015986

PRIDE project URI

• PRIDE
  1. PXD015986
     1. Label: PRIDE project
     2. Name: LC-MS/MS analysis of immunoprecipitation samples of Tetrahymena meiotic proteins Mcmd1 and Pamd1