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PXD015919

PXD015919 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMembrane protein landscape of plant nuclear envelope and nuclear pore complex
DescriptionWe used biochemical fractionation coupled with the subtractive proteomics to profile PNET proteins. Both the crude NE and the total MM fraction were subject to label-free quantitative mass spectrometry (LFQMS). We also adapted proximity labeling-coupled LFQMS (PL-LFQMS) method to map the PNET proteome. Overall, We identified over 200 potential candidates for plant nuclear envelope transmembrane (PNET) proteins and confirmed 14 PNETs.
HostingRepositoryiProX
AnnounceDate2020-03-11
AnnouncementXMLSubmission_2020-11-19_00:29:17.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYu Tang
SpeciesList scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationListN6-biotinyl-L-lysine
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-10-20 03:11:04ID requested
12020-03-10 21:25:49announced
22020-11-19 00:29:18announced2020-11-19: Update publication information
Publication List
Tang Y, Huang A, Gu Y, Global profiling of plant nuclear membrane proteome in Arabidopsis. Nat Plants, 6(7):838-847(2020) [pubmed]
Keyword List
submitter keyword: Arabidopsis seedling, PNET, NE, MM, BioID2, LFQMS, PL-LFQMS
Contact List
Yangnan Gu
contact affiliationDepartment of Plant & Microbial Biology, University of California, Berkeley
contact emailguyangnan@berkeley.edu
lab head
Yu Tang
contact affiliationTsinghua University
contact emailtang414yu@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI