PXD015838-1

PXD015838 is an original dataset announced via ProteomeXchange.

Title	Targeted assay for differentiation and monitoring the concentration of GDF 8 (Myostatin) and GDF11 in mouse serum
Description	Abstract Growth differentiation factor 11 (GDF11) and Myostatin (GDF8) are circulating factor shown to reduce cardiac hypertrophy of aged animals. Although GDF11 and GDF8 have high structural homology, it seems that GDF11 is had a more potent effect on reducing cardiomyocytes size, albeit GDF11 quantification methods have been recently questioned. In this study, we developed the IP based-PRM- LC-MS/MS method for measuring the GDF8 and GDF11 in one assay. The method demonstrated discriminatory power and allow the quantification of both proteins. Comparison of young and old group of mice show declination of both, GDF8 and GDF11 during aging.
HostingRepository	PanoramaPublic
AnnounceDate	2021-01-24
AnnouncementXML	Submission_2021-01-24_16:42:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Olga Shevchuk
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	Carbamidomethyl; Label:13C(6)15N(4)
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-15 15:43:56	ID requested
⏵ 1	2021-01-24 16:42:08	announced

Camparini L, Kollipara L, Sinagra G, Loffredo FS, Sickmann A, Shevchuk O, Targeted Approach to Distinguish and Determine Absolute Levels of GDF8 and GDF11 in Mouse Serum. Proteomics, 20(11):e1900104(2020) [pubmed]

submitter keyword: Parallel Reaction Monitoring (PRM), targeted, quantitative proteomics, Myostatin/GDF8, GDF11

Olga Shevchuk
contact affiliation	ISAS e.V. Otto-Hahn-Straße 6b 44227 Dortmund, Germany
contact email	olga.shevchuk@isas.de
lab head
Olga Shevchuk
contact affiliation	ISAS e.V. Otto-Hahn-Straße 6b 44227 Dortmund, Germany
contact email	olga.shevchuk@isas.de
dataset submitter

Panorama Public dataset URI